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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated PKC Iota Monoclonal antibody

PKC Iota Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat, mouse

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3A1B6

Cat no : CL594-66493

Synonyms

aPKC lambda/iota, DXS1179E, PKC Iota, PKCI, PRKC lambda/iota, PRKCI, Protein kinase C iota type, protein kinase C, iota

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated PKC Iota Monoclonal antibo (CL594-66493)

    IF Staining of HeLa using CL594-66493

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CL594-66493 (PKC iota antibody) at dilution of 1:50.

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66493 targets PKC Iota in IF/ICC applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PKC Iota fusion protein Ag4990 相同性解析による交差性が予測される生物種
Full Name protein kinase C, iota
Calculated molecular weight 68 kDa
GenBank accession numberBC022016
Gene symbol PKC Iota
Gene ID (NCBI) 5584
RRIDAB_2883596
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The atypical protein kinase C isoform PRKC iota (PRKCI) is a member of the protein kinase C (PKC) family of serine/threonine protein kinases. PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. PRKC iota is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. PRKC iota plays a key role in cell proliferation, differentiation, and carcinogenesis, and it has been shown to be a human oncogene.

Protocols

Product Specific Protocols
IF protocol for CL594 PKC Iota antibody CL594-66493Download protocol
FC protocol for CL594 PKC Iota antibody CL594-66493Download protocol
Standard Protocols
Click here to view our Standard Protocols