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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse brain tissue using Biotin-conjugated PKM1-specific Polyclonal antibod (Biotin-15821)

    IHC staining of mouse brain using Biotin-15821

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using Biotin-15821(PKM1-specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Biotin-conjugated PKM1-specific Polyclonal antibod (Biotin-15821)

    IHC staining of mouse brain using Biotin-15821

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using Biotin-15821(PKM1-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using Biotin-conjugated PKM1-specific Polyclonal antibod (Biotin-15821)

    IHC staining of mouse skeletal muscle using Biotin-15821

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using Biotin-15821 (PKM1-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  • Featured Product
  • KD/KO Validated

Biotin-conjugated PKM1-specific Polyclonal antibody

PKM1-specific Polyclonal Antibody for IHC
Cat No. Biotin-15821

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC

Synonyms

PKM1, PK-M1, PKM1 pyruvate kinase, muscle, PKM, Pyruvate kinase PKM

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Biotin
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inmouse brain tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-15821 targets PKM1-specific in IHC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name PKM pyruvate kinase, muscle
Calculated molecular weight 58 kDa
Observed molecular weight 58 kDa
GenBank accession numberNM_182470
Gene Symbol PKM
Gene ID (NCBI) 5315
RRIDAB_2883058
Conjugate Biotin
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP14618
Storage Buffer PBS with 50% Glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PKM, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms named PKM1 and PKM2. The primary pyruvate kinase isoform before tumour development is PK-M1; however, the primary isoform from four independent tumours is PK-M2 (PMID:18337823). This antibody is conjugated with Biotin and it's specific to PKM1 isoform.

Protocols

Product Specific Protocols
IHC protocol for Biotin PKM1-specific antibody Biotin-15821Download protocol
Standard Protocols
Click here to view our Standard Protocols