CoraLite® Plus 488-conjugated PLA2G4A Monoclonal antibody
PLA2G4A Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
Human
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2D10H8
Cat no : CL488-68133
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-68133 targets PLA2G4A in IF/ICC applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | PLA2G4A fusion protein Ag32674 相同性解析による交差性が予測される生物種 |
| Full Name | phospholipase A2, group IVA (cytosolic, calcium-dependent) |
| Calculated molecular weight | 749 aa, 85 kDa |
| Observed molecular weight | 85-100 kDa |
| GenBank accession number | BC114340 |
| Gene symbol | PLA2G4A |
| Gene ID (NCBI) | 5321 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PLA2G4A, also named cPLA2 (cytosolic phospholipase A2), is a highly conserved and widely expressed enzyme that can promote lipid mediator production. It is present in the cytosol of resting cells and upon activation by a variety of agonists, undergoes Ca++-directed translocation to perinuclear membranes.PLA2G4A plays a major role in membrane lipid remodeling and biosynthesis of lipid mediators of the inflammatory response. It has been reported that the activity and expression levels of PLA2G4A are upregulated in several neurodegenerative diseases. It is a 749 amino acid, 85 kDa protein that migrates at 100-112 kDa in polyacrylamide gels(PMID: 17459165).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 PLA2G4A antibody CL488-68133 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
