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Tested Applications
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-66752 targets PLA2G4D in IF/ICC applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | PLA2G4D fusion protein Ag4178 相同性解析による交差性が予測される生物種 |
| Full Name | phospholipase A2, group IVD (cytosolic) |
| Calculated molecular weight | 818 aa, 92 kDa |
| Observed molecular weight | 92 kDa |
| GenBank accession number | BC034571 |
| Gene Symbol | PLA2G4D |
| Gene ID (NCBI) | 283748 |
| RRID | AB_2934493 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q86XP0 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PLA2G4D(Cytosolic phospholipase A2 delta) belongs to the phospholipase A2 enzyme family,catalyzes the hydrolysis of glycerophospholipids at the sn-2 position and then liberate free fatty acids and lysophospholipids.PLA2G4D expression is specifically upregulated in the upper epidermis of patients with inflammatory skin diseases(PMID:14709560).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 PLA2G4D antibody CL488-66752 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |