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  • KD/KO Validated

PAFAH Polyclonal antibody

PAFAH Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15526-1-AP

Synonyms

PLA2G7, gVIIA-PLA2, gVIIA PLA2, Group-VIIA phospholipase A2, EC:3.1.1.47


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Tested Applications

Positive WB detected inRAW 264.7 cells, mouse spleen tissue, mouse liver tissue, RAW264.7
Positive IHC detected inhuman tonsillitis tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15526-1-AP targets PAFAH in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PAFAH fusion protein Ag7227 相同性解析による交差性が予測される生物種
Full Name phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma)
Calculated molecular weight 50 kDa
Observed molecular weight 43-67 kDa
GenBank accession numberBC038452
Gene symbol PLA2G7
Gene ID (NCBI) 7941
RRIDAB_2878146
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PAFAH(platelet-activating factor acetylhydrolase, plasma) is also named as PLA2G7(phospholipase A2, group VII), Lp-PLA2 (Lipoprotein-associated phospholipase A2), LDL-PLA2, LDL-PLA and belongs to the AB hydrolase superfamily and lipase family. It is an enzyme which catalyzes the hydrolysis of acetyl ester at the sn-2 position of PAF. The family of PAF-Ahs consists of two intracellular isoforms (Ib and II), and one secreted isoform (plasma). Plasma PAF-AH is a monomeric polypeptide with a molecular weight of 45 kDa and it can show broad bands with a molecular weight of 43-67 kDa that it can be extensively N-glycosylated(PMID:12547653). Defects in PLA2G7 are the cause of platelet-activating factor acetylhydrolase deficiency (PAFAD), susceptibility to asthma (ASTHMA), susceptibility to atopic hypersensitivity (ATOPY).

Protocols

Product Specific Protocols
WB protocol for PAFAH antibody 15526-1-APDownload protocol
IHC protocol for PAFAH antibody 15526-1-APDownload protocol
IF protocol for PAFAH antibody 15526-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Cell Biol

Stromal-epithelial crosstalk regulates kidney progenitor cell differentiation.

Authors - Das Amrita A
mouseWB

Nat Commun

VDAC2 enables BAX to mediate apoptosis and limit tumor development.

Authors - Hui San Chin
human,mouseIF,IHC,WB

J Immunother Cancer

Inhibiting PLA2G7 reverses the immunosuppressive function of intratumoral macrophages and augments immunotherapy response in hepatocellular carcinoma

Authors - Feng Zhang
mouseWB

J Extracell Vesicles

Characterization of protein, long noncoding RNA and microRNA signatures in extracellular vesicles derived from resting and degranulated mast cells.

Authors - Yuting Liang
mouseIF,WB

Nat Metab

Elevated dietary ω-6 polyunsaturated fatty acids induce reversible peripheral nerve dysfunction that exacerbates comorbid pain conditions.

Authors - Jacob T Boyd
  • KD Validated
humanIF

Cell Death Discov

Characterizing cellular heterogeneity in fibrotic hypersensitivity pneumonitis by single-cell transcriptional analysis.

Authors - Junyi Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Sathyanarayani (Verified Customer) (05-12-2023)

I used this PAFAH antibody at a range of different dilutions from 1:300 to 1:1000 but unfortunately I could not detect any bands from this antibody when I used lysed mouse blood. The loading controls which were also from Proteintech produced strong clear bands on the western blot.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:300
  • Cell Tissue Type: Lysed mouse blood