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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using CoraLite® Plus 488-conjugated PAFAH Polyclonal ant (CL488-15526)

    IF Staining of RAW 264.7 using CL488-15526

    Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using CoraLite® Plus 488 PAFAH antibody (CL488-15526) at dilution of 1:200.

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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated PAFAH Polyclonal antibody

PAFAH Polyclonal Antibody for IF/ICC
Cat No. CL488-15526

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Synonyms

Group VIIA phospholipase A2, gVIIA PLA2, LDL PLA(2), LDL PLA2, PAF 2 acylhydrolase, PAF acetylhydrolase, PAFAH, PLA2G7

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-15526 targets PAFAH in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PAFAH fusion protein Ag7227 相同性解析による交差性が予測される生物種
Full Name phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma)
Calculated molecular weight 50 kDa
Observed molecular weight 43-67 kDa
GenBank accession numberBC038452
Gene Symbol PLA2G7
Gene ID (NCBI) 7941
RRIDAB_3672633
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen Affinity Purified
UNIPROT IDQ13093
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PAFAH(platelet-activating factor acetylhydrolase, plasma) is also named as PLA2G7(phospholipase A2, group VII), Lp-PLA2 (Lipoprotein-associated phospholipase A2), LDL-PLA2, LDL-PLA and belongs to the AB hydrolase superfamily and lipase family. It is an enzyme which catalyzes the hydrolysis of acetyl ester at the sn-2 position of PAF. The family of PAF-Ahs consists of two intracellular isoforms (Ib and II), and one secreted isoform (plasma). Plasma PAF-AH is a monomeric polypeptide with a molecular weight of 45 kDa and it can show broad bands with a molecular weight of 43-67 kDa that it can be extensively N-glycosylated(PMID:12547653). Defects in PLA2G7 are the cause of platelet-activating factor acetylhydrolase deficiency (PAFAD), susceptibility to asthma (ASTHMA), susceptibility to atopic hypersensitivity (ATOPY).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 PAFAH antibody CL488-15526Download protocol
Standard Protocols
Click here to view our Standard Protocols