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  • Featured Product
  • KD/KO Validated

PLAA Polyclonal antibody, PBS Only

PLAA Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12529-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using PLAA Polyclonal antibody (12529-1-AP)

    WB analysis of MCF-7 using 12529-1-AP (same clone as 12529-1-PBS)

    WB result of PLAA antibody (12529-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLAA transfected MCF-7 cells. This data was developed using the same antibody clone with 12529-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using PLAA Polyclonal antibody (12529-1-AP)

    WB analysis of K-562 using 12529-1-AP (same clone as 12529-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 12529-1-AP (PLAA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12529-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using PLAA Polyclonal antibody (12529-1-AP)

    WB analysis of MCF-7 using 12529-1-AP (same clone as 12529-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 12529-1-AP (PLAA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12529-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of K-562 cells using PLAA Polyclonal antibody (12529-1-AP)

    IP experiment of K-562 using 12529-1-AP (same clone as 12529-1-PBS)

    IP result of anti-PLAA (IP:12529-1-AP, 4ug; Detection:12529-1-AP 1:600) with K-562 cells lysate 3200ug. This data was developed using the same antibody clone with 12529-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PLAA Polyclonal antibody (12529-1-AP)

    IHC staining of human lung cancer using 12529-1-AP (same clone as 12529-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12529-1-AP (PLAA Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12529-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PLAA Polyclonal antibody (12529-1-AP)

    IHC staining of human lung cancer using 12529-1-AP (same clone as 12529-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12529-1-AP (PLAA Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12529-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PLAA Polyclonal antibody (12529-1-AP)

    IHC staining of human kidney using 12529-1-AP (same clone as 12529-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12529-1-AP (PLAA Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12529-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PLAA Polyclonal antibody (12529-1-AP)

    IHC staining of human kidney using 12529-1-AP (same clone as 12529-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12529-1-AP (PLAA Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12529-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12529-1-PBS targets PLAA in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLAA fusion protein Ag3224 相同性解析による交差性が予測される生物種
Full Name phospholipase A2-activating protein
Calculated molecular weight 738 aa, 81 kDa
Observed molecular weight 72-74 kDa
GenBank accession numberBC032551
Gene symbol PLAA
Gene ID (NCBI) 9373
RRIDAB_2164881
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Phospholipase A-2-activating protein (PLAA), also named PLAP, can interact with ubiquitin and is involved in the maintenance of ubiquitin levels. PLAA contains multiple consensus domains including WD repeats, PFU domain and PUL domain. Although the function of PLAA remains unclear, ubiquitin binding of PLAA might be the central role that connects ubiquitination and degradation in ERAD. Recent finding revealed that PLAA served as a novel nociveptive mediator after incision, and its expression level is regulated by miR-203.