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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using PLAA Monoclonal antibody (60244-1-Ig)

    WB analysis of MCF-7 using 60244-1-Ig

    WB result of PLAA antibody (60244-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLAA transfected MCF-7 cells.

  • Western Blot (WB) analysis of ROS1728 cells using PLAA Monoclonal antibody (60244-1-Ig)

    WB analysis of ROS1728 using 60244-1-Ig

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 60244-1-Ig (PLAA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using PLAA Monoclonal antibody (60244-1-Ig)

    WB analysis of A431 using 60244-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 60244-1-Ig (PLAA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using PLAA Monoclonal antibody (60244-1-Ig)

    WB analysis of K-562 using 60244-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 60244-1-Ig (PLAA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using PLAA Monoclonal antibody (60244-1-Ig)

    WB analysis of MCF-7 using 60244-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 60244-1-Ig (PLAA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PLAA Monoclonal antibody (60244-1-Ig)

    IHC staining of human kidney using 60244-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60244-1-Ig (PLAA Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PLAA Monoclonal antibody (60244-1-Ig)

    IHC staining of human kidney using 60244-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60244-1-Ig (PLAA Antibody) at dilution of 1:200 (under 40x lens).

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  • KD/KO Validated

PLAA Monoclonal antibody

PLAA Monoclonal Antibody for WB, IHC, ELISA
Cat No. 60244-1-Ig
Clone No.5C10G9

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

DOA1, PLA2P, PLAA, PLAP

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inROS1728 cells, K-562 cells, MCF-7 cells, A431 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60244-1-Ig targets PLAA in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PLAA fusion protein Ag3224 相同性解析による交差性が予測される生物種
Full Name phospholipase A2-activating protein
Calculated molecular weight 738 aa, 81 kDa
Observed molecular weight 75-80 kDa
GenBank accession numberBC032551
Gene Symbol PLAA
Gene ID (NCBI) 9373
RRIDAB_2881366
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y263
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Phospholipase A-2-activating protein (PLAA), also named PLAP, can interact with ubiquitin and is involved in the maintenance of ubiquitin levels. PLAA contains multiple consensus domains including WD repeats, PFU domain and PUL domain. Although the function of PLAA remains unclear, ubiquitin binding of PLAA might be the central role that connects ubiquitination and degradation in ERAD. Recent finding revealed that PLAA served as a novel nociveptive mediator after incision, and its expression level is regulated by miR-203.

Protocols

Product Specific Protocols
WB protocol for PLAA antibody 60244-1-IgDownload protocol
IHC protocol for PLAA antibody 60244-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols