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  • Featured Product
  • KD/KO Validated

PLAA Monoclonal antibody, PBS Only

PLAA Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5C10G9

Cat no : 60244-1-PBS

Synonyms

DOA1, PLA2P, PLAA, PLAP

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using PLAA Monoclonal antibody (60244-1-Ig)

    WB analysis of MCF-7 using 60244-1-Ig (same clone as 60244-1-PBS)

    WB result of PLAA antibody (60244-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLAA transfected MCF-7 cells. This data was developed using the same antibody clone with 60244-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of ROS1728 cells using PLAA Monoclonal antibody (60244-1-Ig)

    WB analysis of ROS1728 using 60244-1-Ig (same clone as 60244-1-PBS)

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 60244-1-Ig (PLAA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60244-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using PLAA Monoclonal antibody (60244-1-Ig)

    WB analysis of K-562 using 60244-1-Ig (same clone as 60244-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 60244-1-Ig (PLAA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60244-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using PLAA Monoclonal antibody (60244-1-Ig)

    WB analysis of MCF-7 using 60244-1-Ig (same clone as 60244-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 60244-1-Ig (PLAA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60244-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using PLAA Monoclonal antibody (60244-1-Ig)

    WB analysis of A431 using 60244-1-Ig (same clone as 60244-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 60244-1-Ig (PLAA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60244-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PLAA Monoclonal antibody (60244-1-Ig)

    IHC staining of human kidney using 60244-1-Ig (same clone as 60244-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60244-1-Ig (PLAA Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60244-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PLAA Monoclonal antibody (60244-1-Ig)

    IHC staining of human kidney using 60244-1-Ig (same clone as 60244-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60244-1-Ig (PLAA Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60244-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60244-1-PBS targets PLAA in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PLAA fusion protein Ag3224 相同性解析による交差性が予測される生物種
Full Name phospholipase A2-activating protein
Calculated molecular weight 738 aa, 81 kDa
Observed molecular weight 75-80 kDa
GenBank accession numberBC032551
Gene symbol PLAA
Gene ID (NCBI) 9373
RRIDAB_2881366
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Phospholipase A-2-activating protein (PLAA), also named PLAP, can interact with ubiquitin and is involved in the maintenance of ubiquitin levels. PLAA contains multiple consensus domains including WD repeats, PFU domain and PUL domain. Although the function of PLAA remains unclear, ubiquitin binding of PLAA might be the central role that connects ubiquitination and degradation in ERAD. Recent finding revealed that PLAA served as a novel nociveptive mediator after incision, and its expression level is regulated by miR-203.