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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PLD1 Polyclonal antibody (18355-1-AP)

    WB analysis using 18355-1-AP (same clone as 18355-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18355-1-AP (PLD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18355-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using PLD1 Polyclonal antibody (18355-1-AP)

    IHC staining of mouse heart using 18355-1-AP (same clone as 18355-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18355-1-AP (PLD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18355-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PLD1 Polyclonal antibody (18355-1-AP)

    IF Staining of HeLa using 18355-1-AP (same clone as 18355-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PLD1 antibody (18355-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18355-1-PBS in a different storage buffer formulation.

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PLD1 Polyclonal antibody, PBS Only

PLD1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 18355-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18355-1-PBS targets PLD1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLD1 fusion protein Ag13227 相同性解析による交差性が予測される生物種
Full Name phospholipase D1, phosphatidylcholine-specific
Calculated molecular weight 1074 aa, 124 kDa
Observed molecular weight112-124 kDa
GenBank accession numberBC068976
Gene Symbol PLD1
Gene ID (NCBI) 5337
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13393
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

PLD1(Phospholipase D1) belongs to the phospholipase D family. It is implicated in the release process of a variety of hormones, most likely at a late stage of exocytosis through the production of lipids that are essential for the fusion process and seem to be also essential for fast neurotransmitter release. PLD1 is primarily localized in vesicular structures such as endosomes, lysosomes, and autophagosomes.