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KD/KO Validated

PLEK2 Polyclonal antibody

Name: PLEK2 Polyclonal antibody
Brand: Proteintech
SKU: 11685-1-AP

PLEK2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11685-1-AP

Synonyms

Pleckstrin-2, Pleckstrin 2

Validation Data Gallery

Filter:

AllWBIHCIF/ICCFC (Intra)

WB analysis using 11685-1-AP
U2OS (positive), Jurkat, Ramos, and U-937 (negative) cells were subjected to SDS PAGE followed by western blot with 11685-1-AP (PLEK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis using 11685-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11685-1-AP (PLEK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Caco-2 using 11685-1-AP
Caco-2 cells were subjected to SDS PAGE followed by western blot with 11685-1-AP (PLEK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human pancreas cancer using 11685-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11685-1-AP (PLEK2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of mouse spleen using 11685-1-AP
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 11685-1-AP (PLEK2 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of mouse spleen using 11685-1-AP
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 11685-1-AP (PLEK2 Antibody) at dilution of 1:200 (under 40x lens).
IF Staining of HT-29 using 11685-1-AP
Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using PLEK2 antibody (11685-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
FC experiment of HT-29 using 11685-1-AP
1X10^6 HT-29 cells were intracellularly stained with 0.4 ug Anti-Human PLEK2 (11685-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 11685-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HT-29 cells, U2OS cells, Caco-2 cells, PC-3 cells
Positive IHC detected in	human pancreas cancer tissue, mouse spleen tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HT-29 cells
Positive FC (Intra) detected in	HT-29 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 10 publications below
WB	See 15 publications below
IHC	See 9 publications below
IF	See 3 publications below
IP	See 1 publications below
CoIP	See 2 publications below

Product Information

11685-1-AP targets PLEK2 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PLEK2 fusion protein Ag2297 相同性解析による交差性が予測される生物種
Full Name	pleckstrin 2
Calculated molecular weight	353 aa, 40 kDa
Observed molecular weight	38-40 kDa
GenBank accession number	BC001226
Gene symbol	PLEK2
Gene ID (NCBI)	26499
RRID	AB_2252364
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for PLEK2 antibody 11685-1-AP	Download protocol
IHC protocol for PLEK2 antibody 11685-1-AP	Download protocol
IF protocol for PLEK2 antibody 11685-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB,IHC	J Clin Invest Loss of pleckstrin-2 reverts lethality and vascular occlusions in JAK2V617F-positive myeloproliferative neoplasms. Authors - Baobing Zhao KO Validated View Article
human	WB,IHC	Cancer Lett Pleckstrin-2 promotes tumour immune escape from NK cells by activating the MT1-MMP-MICA signalling axis in gastric cancer Authors - Deli Mao KD Validated View Article
human	WB	Am J Pathol Pleckstrin-2 Mediates the Activation of AKT in Prostate Cancer and Is Repressed by Androgen Receptor Authors - Xu Han View Article
mouse	WB,IF	Haematologica Targeted shRNA screening identified critical roles of pleckstrin-2 in erythropoiesis. Authors - Baobing Zhao KD Validated View Article
human	WB,IHC,IF	Mol Carcinog PLEK2 mediates metastasis and invasion via α5-nAChR activation in nicotine-induced lung adenocarcinoma Authors - Qiang Li KD Validated View Article
mouse	WB, IF	Commun Biol Pleckstrin-2 is essential for erythropoiesis in β-thalassemic mice, reducing apoptosis and enhancing enucleation. Authors - Maria Feola View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PLEK2 Polyclonal antibody

PLEK2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Validation Data Gallery

WB analysis using 11685-1-AP

WB analysis using 11685-1-AP

WB analysis of Caco-2 using 11685-1-AP

IHC staining of human pancreas cancer using 11685-1-AP

IHC staining of mouse spleen using 11685-1-AP

IHC staining of mouse spleen using 11685-1-AP

IF Staining of HT-29 using 11685-1-AP

FC experiment of HT-29 using 11685-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Loss of pleckstrin-2 reverts lethality and vascular occlusions in JAK2V617F-positive myeloproliferative neoplasms.

Pleckstrin-2 promotes tumour immune escape from NK cells by activating the MT1-MMP-MICA signalling axis in gastric cancer

Pleckstrin-2 Mediates the Activation of AKT in Prostate Cancer and Is Repressed by Androgen Receptor

Targeted shRNA screening identified critical roles of pleckstrin-2 in erythropoiesis.

PLEK2 mediates metastasis and invasion via α5-nAChR activation in nicotine-induced lung adenocarcinoma

Pleckstrin-2 is essential for erythropoiesis in β-thalassemic mice, reducing apoptosis and enhancing enucleation.