• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated PLEK2 Polyclonal antibody

PLEK2 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-11685

Synonyms

Pleckstrin-2, Pleckstrin 2

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HT-29 cells using CoraLite®594-conjugated PLEK2 Polyclonal antibody (CL594-11685)

    IF Staining of HT-29 using CL594-11685

    Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using CoraLite®594 PLEK2 antibody (CL594-11685) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HT-29 cells using CoraLite®594-conjugated PLEK2 Polyclonal antibody (CL594-11685)

    FC experiment of HT-29 using CL594-11685

    1X10^6 HT-29 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human PLEK2 (CL594-11685) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

☆大好評トライアルサイズ ¥20,000～！ >>> 一次抗体特別価格キャンペーン開催中！

Tested Applications

Positive IF/ICC detected inHT-29 cells
Positive FC (Intra) detected inHT-29 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-11685 targets PLEK2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLEK2 fusion protein Ag2297 相同性解析による交差性が予測される生物種
Full Name pleckstrin 2
Calculated molecular weight 353 aa, 40 kDa
Observed molecular weight 38-40 kDa
GenBank accession numberBC001226
Gene symbol PLEK2
Gene ID (NCBI) 26499
RRIDAB_2919771
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL594 PLEK2 antibody CL594-11685Download protocol
Standard Protocols
Click here to view our Standard Protocols