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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using PLK2 Recombinant antibody (83431-1-RR)

    IF Staining of hTERT-RPE1 using 83431-1-RR (same clone as 83431-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using PLK2 antibody (83431-1-RR, Clone: 230520B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Acetyl-Tubulin (Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red). This data was developed using the same antibody clone with 83431-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A549 cells using PLK2 Recombinant antibody (83431-1-RR)

    FC experiment of A549 using 83431-1-RR (same clone as 83431-1-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug Plk2 Recombinant Antibody (83431-1-RR, Clone:230520B2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83431-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83431-1-RR

    Affinity and Kinetic Characterization of 83431-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83431-1-RR against Human PLK2 were performed. The affinity constant is 1.53 nM.

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PLK2 Recombinant antibody, PBS Only

PLK2 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83431-1-PBS
Clone No.230520B2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

PLK-2, PLK 2, hSNK, hPlk2, EC:2.7.11.21

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83431-1-PBS targets PLK2 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PLK2 fusion protein Ag29837 相同性解析による交差性が予測される生物種
Full Name polo-like kinase 2 (Drosophila)
Calculated molecular weight 685 aa, 78 kDa
GenBank accession numberBC013879
Gene Symbol PLK2
Gene ID (NCBI) 10769
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9NYY3
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.