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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PLK2 Recombinant antibody

PLK2 Recombinant Antibody for IF, ELISA, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF, ELISA, FC (Intra)

Conjugate

Unconjugated

CloneNo.

230520B2

Cat no : 83431-1-RR

Synonyms

PLK 2, PLK2, Polo like kinase 2, Serum inducible kinase, SNK

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using PLK2 Recombinant antibody (83431-1-RR)

    IF Staining of hTERT-RPE1 using 83431-1-RR

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using PLK2 antibody (83431-1-RR, Clone: 230520B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), Acetyl-Tubulin (Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

  • Flow cytometry (FC) experiment of A549 cells using PLK2 Recombinant antibody (83431-1-RR)

    FC experiment of A549 using 83431-1-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug Plk2 Recombinant Antibody (83431-1-RR, Clone:230520B2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity experiments of 83431-1-RR

    Affinity experiments of 83431-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83431-1-RR against Human PLK2 were performed. The affinity constant is 1.53 nM.

Tested Applications

Positive IF detected inhTERT-RPE1 cells
Positive FC detected inA549 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83431-1-RR is PLK2 Fusion Protein expressed in E. coli.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PLK2 fusion protein Ag29837 相同性解析による交差性が予測される生物種
Full Name polo-like kinase 2 (Drosophila)
Calculated molecular weight 685 aa, 78 kDa
GenBank accession numberBC013879
Gene symbol PLK2
Gene ID (NCBI) 10769
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PLK2(Polo-like kinase 2) is also named as SNK and belongs to the Ser/Thr protein kinase family. It plays a role in normal cell division. Plk2 expression is detected only in G1, and endogenous Plk2 is rapidly turned over. Therefore, the expression of the three mammalian Plks during the cell cycle resembles the successive transition of the cyclins, prompting speculation that Plks may reg-ulate the cell cycle in a manner analogous to that of Cdks(PMID:12972611).

Protocols

Product Specific Protocols
IF protocol for PLK2 antibody 83431-1-RRDownload protocol
FC protocol for PLK2 antibody 83431-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols