Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis of A431 using 21214-1-AP
WB result of PLOD2-Specific antibody (21214-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLOD2-Specific transfected A431 cells.
WB analysis of THLE-2 using 21214-1-AP
WB result of PLOD2-Specific antibody (21214-1-AP 1:3000; room temperature for 1.5 hours) with wild-type and PLOD2 knockout THLE-2 cells.
WB analysis using 21214-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21214-1-AP (PLOD2-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis using 21214-1-AP
DU 145 cells lysates were subjected to SDS PAGE followed by western blot with 21214-1-AP (PLOD2-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of HT-1080 using 21214-1-AP
HT-1080 cells were subjected to SDS PAGE followed by western blot with 21214-1-AP (PLOD2-Specific antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of mouse testis using 21214-1-AP
IP result of anti-PLOD2-Specific (IP:21214-1-AP, 4ug; Detection:21214-1-AP 1:500) with mouse testis tissue lysate 5000ug.
IHC staining of human liver cancer using 21214-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21214-1-AP (PLOD2-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 21214-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21214-1-AP (PLOD2-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skeletal muscle using 21214-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 21214-1-AP (PLOD2-Specific antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human skeletal muscle using 21214-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 21214-1-AP (PLOD2-Specific antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human pancreas using 21214-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 21214-1-AP (PLOD2-Specific antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human pancreas using 21214-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 21214-1-AP (PLOD2-Specific antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HeLa using 21214-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PLOD2-Specific antibody (21214-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
FC experiment of HeLa using 21214-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PLOD2-Specific (21214-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO Validated

PLOD2-Specific Polyclonal antibody

PLOD2-Specific Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 21214-1-AP

Publications(50)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

PLOD2, Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2, PLOD2/LH2, Lysyl hydroxylase 2, EC:1.14.11.4

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 647 PE

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (50)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Caco-2 cells, A431 cells, DU 145 cells, HT-1080 cells, HEK-293 cells, HeLa cells, HepG2 cells
Positive IP detected in	mouse testis tissue
Positive IHC detected in	human liver cancer tissue, human pancreas tissue, human skeletal muscle tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 11 publications below
WB	See 36 publications below
IHC	See 21 publications below
IF	See 7 publications below

Product Information

21214-1-AP targets PLOD2-Specific in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rabbit, zebrafish
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PLOD2-Specific fusion protein Ag15614 相同性解析による交差性が予測される生物種
Full Name	procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2
Calculated molecular weight	758 aa, 85 kDa
Observed molecular weight	87 kDa
GenBank accession number	BC037169
Gene Symbol	PLOD2
Gene ID (NCBI)	5352
RRID	AB_10733347
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O00469
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PLOD2,also named as LH2, forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. It is a potential novel prognostic factor for HCC patients following surgery. Among the PLOD genes, PLOD2 contributes to cancer prognosis and angiogenesis. Several authors have reported that PLOD2 expression might provide prognostic information about malignant tumours such as glioblastoma. PLOD2 expression is a useful biomarker for the effects of antiangiogenic treatment for malignancy. (PMID:22098155). It has 2 isoforms produced by alternative splicing and seven glycosylation sites.This antibody is specific to PLOD2.

Protocols

Product Specific Protocols
WB protocol for PLOD2-Specific antibody 21214-1-AP	Download protocol
IHC protocol for PLOD2-Specific antibody 21214-1-AP	Download protocol
IF protocol for PLOD2-Specific antibody 21214-1-AP	Download protocol
IP protocol for PLOD2-Specific antibody 21214-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB, IHC	J Clin Invest Lysyl hydroxylase 2 induces a collagen cross-link switch in tumor stroma. Authors - Yulong Chen View Article
zebrafish	WB	Cancer Res Sarcoma Cells Secrete Hypoxia-Modified Collagen VI to Weaken the Lung Endothelial Barrier and Promote Metastasis Authors - Ying Liu View Article
human, mouse	WB	Cancer Discov Hypoxia-Dependent Modification of Collagen Networks Promotes Sarcoma Metastasis. Authors - Eisinger-Mathason T S Karin TS View Article
mouse	WB	Arthritis Rheumatol Therapeutic effect of cyclin-dependent kinase 4/6 inhibitor on dermal fibrosis in murine models of systemic sclerosis. Authors - Akio Yamamoto View Article
human	WB	Proc Natl Acad Sci U S A Intratumoral oxygen gradients mediate sarcoma cell invasion. Authors - Daniel M Lewis View Article
mouse	IHC	Arthritis Rheum Gene expression analysis of osteoarthritis synovium reveals elevation of transforming growth factor-beta responsive genes in osteoarthritis-related fibrosis. Authors - Remst D F G DF View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of A431 using 21214-1-AP

WB analysis of THLE-2 using 21214-1-AP

WB analysis using 21214-1-AP

WB analysis using 21214-1-AP

WB analysis of HT-1080 using 21214-1-AP

IP experiment of mouse testis using 21214-1-AP

IHC staining of human liver cancer using 21214-1-AP

IHC staining of human liver cancer using 21214-1-AP

IHC staining of human skeletal muscle using 21214-1-AP

IHC staining of human skeletal muscle using 21214-1-AP

IHC staining of human pancreas using 21214-1-AP

IHC staining of human pancreas using 21214-1-AP

IF Staining of HeLa using 21214-1-AP

FC experiment of HeLa using 21214-1-AP

PLOD2-Specific Polyclonal antibody

PLOD2-Specific Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Lysyl hydroxylase 2 induces a collagen cross-link switch in tumor stroma.

Sarcoma Cells Secrete Hypoxia-Modified Collagen VI to Weaken the Lung Endothelial Barrier and Promote Metastasis

Hypoxia-Dependent Modification of Collagen Networks Promotes Sarcoma Metastasis.

Therapeutic effect of cyclin-dependent kinase 4/6 inhibitor on dermal fibrosis in murine models of systemic sclerosis.

Intratumoral oxygen gradients mediate sarcoma cell invasion.

Gene expression analysis of osteoarthritis synovium reveals elevation of transforming growth factor-beta responsive genes in osteoarthritis-related fibrosis.