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Validation Data Gallery

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  • Flow cytometry (FC) experiment of HeLa cells using PE-conjugated PLOD2-Specific Polyclonal antibody (PE-21214)

    FC experiment of HeLa using PE-21214

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug PE-conjugated PLOD2-Specific Polyclonal antibody (PE-21214)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  • KD/KO Validated

PE-conjugated PLOD2-Specific Polyclonal antibody

PLOD2-Specific Polyclonal Antibody for FC (Intra)
Cat No. PE-21214

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Synonyms

PLOD2, Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2, PLOD2/LH2, Lysyl hydroxylase 2, EC:1.14.11.4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  PE
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

PE-21214 targets PLOD2-Specific in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLOD2-Specific fusion protein Ag15614 相同性解析による交差性が予測される生物種
Full Name procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2
Calculated molecular weight 758 aa, 85 kDa
Observed molecular weight 87 kDa
GenBank accession numberBC037169
Gene Symbol PLOD2
Gene ID (NCBI) 5352
RRIDAB_3673988
Conjugate PE Fluorescent Dye
Excitation/Emission maxima wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00469
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

PLOD2, also named as LH2, forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. It is a potential novel prognostic factor for HCC patients following surgery. Among the PLOD genes, PLOD2 contributes to cancer prognosis and angiogenesis. Several authors have reported that PLOD2 expression might provide prognostic information about malignant tumours such as glioblastoma. PLOD2 expression is a useful biomarker for the effects of antiangiogenic treatment for malignancy. (PMID:22098155). It has 2 isoforms produced by alternative splicing and seven glycosylation sites.This antibody is specific to PLOD2.

Protocols

Product Specific Protocols
FC protocol for PE PLOD2-Specific antibody PE-21214Download protocol
Standard Protocols
Click here to view our Standard Protocols