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PLOD3 Polyclonal antibody

PLOD3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 11027-1-AP

Synonyms

Procollagen glucosyltransferase, Procollagen galactosyltransferase, Multifunctional procollagen lysine hydroxylase and glycosyltransferase LH3, Lysyl hydroxylase 3, LH3


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Tested Applications

Positive WB detected inA549 cells, PC-3 cells, HepG2 cells, HeLa cells, human placenta tissue, mouse placenta tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman pancreas cancer tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11027-1-AP targets PLOD3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, drosophila
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLOD3 fusion protein Ag1480 相同性解析による交差性が予測される生物種
Full Name procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3
Calculated molecular weight 738 aa, 85 kDa
Observed molecular weight 80-85 kDa
GenBank accession numberBC011674
Gene symbol PLOD3
Gene ID (NCBI) 8985
RRIDAB_2165781
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PLOD3, also named as LH3, forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links. The major function of PLOD3 in osteoblasts is to glucosylate galactosylhydroxylysine residues in type I collagen.

Protocols

Product Specific Protocols
WB protocol for PLOD3 antibody 11027-1-APDownload protocol
IHC protocol for PLOD3 antibody 11027-1-APDownload protocol
IF protocol for PLOD3 antibody 11027-1-APDownload protocol
IP protocol for PLOD3 antibody 11027-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Adv

p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi.

Authors - Xiaochao Tan

Nat Commun

Molecular architecture of the multifunctional collagen lysyl hydroxylase and glycosyltransferase LH3.

Authors - Luigi Scietti
humanWB,IF

Nat Commun

Regulation of post-Golgi LH3 trafficking is essential for collagen homeostasis.

Authors - Blerida Banushi
humanWB

Am J Hum Genet

A connective tissue disorder caused by mutations of the lysyl hydroxylase 3 gene.

Authors - Salo Antti M AM
mouseWB

PLoS Genet

Abnormal type I collagen post-translational modification and crosslinking in a cyclophilin B KO mouse model of recessive osteogenesis imperfecta.

Authors - Wayne A Cabral
humanWB

Matrix Biol

Mutations in PLOD3, encoding lysyl hydroxylase 3, cause a complex connective tissue disorder including recessive dystrophic epidermolysis bullosa-like blistering phenotype with abnormal anchoring fibrils and type VII collagen deficiency.

Authors - Hassan Vahidnezhad