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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PMS1 Recombinant antibody, PBS Only

PMS1 Recombinant Antibody for ELISA, IF/ICC, WB

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

ELISA, IF/ICC, WB

Conjugate

Unconjugated

CloneNo.

240408D9

Cat no : 83456-6-PBS

Synonyms

PMSL1, MLH2, hPMS1, HNPCC3, 240408D9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PMS1 Recombinant antibody (83456-6-RR)

    WB analysis using 83456-6-RR (same clone as 83456-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83456-6-RR (PMS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83456-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using PMS1 Recombinant antibody (83456-6-RR)

    IF Staining of Caco-2 using 83456-6-RR (same clone as 83456-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using PMS1 antibody (83456-6-RR, Clone: 240408D9 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83456-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PMS1 Recombinant antibody (83456-6-RR)

    IF Staining of HeLa using 83456-6-RR (same clone as 83456-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PMS1 antibody (83456-6-RR, Clone: 240408D9 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83456-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using PMS1 Recombinant antibody (83456-6-RR)

    IF Staining of Caco-2 using 83456-6-RR (same clone as 83456-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using PMS1 antibody (83456-6-RR, Clone: 240408D9 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83456-6-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using PMS1 Recombinant antibody (83456-6-RR)

    FC experiment of HeLa using 83456-6-RR (same clone as 83456-6-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug PMS1 Recombinant antibody (83456-6-RR, Clone:240408D9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83456-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83456-6-RR

    Affinity and Kinetic Characterization of 83456-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83456-6-RR against Human PMS1 were performed. The affinity constant is 0.367 nM.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83456-6-PBS is PMS1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PMS1 fusion protein Ag28132 相同性解析による交差性が予測される生物種
Full Name PMS1 postmeiotic segregation increased 1 (S. cerevisiae)
GenBank accession numberBC096331
Gene symbol PMS1
Gene ID (NCBI) 5378
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.