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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PMS1 Recombinant antibody

PMS1 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240408D9

Cat no : 83456-6-RR

Synonyms

PMSL1, MLH2, hPMS1, HNPCC3, 240408D9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PMS1 Recombinant antibody (83456-6-RR)

    WB analysis using 83456-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83456-6-RR (PMS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using PMS1 Recombinant antibody (83456-6-RR)

    IF Staining of Caco-2 using 83456-6-RR

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using PMS1 antibody (83456-6-RR, Clone: 240408D9 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PMS1 Recombinant antibody (83456-6-RR)

    IF Staining of HeLa using 83456-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PMS1 antibody (83456-6-RR, Clone: 240408D9 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using PMS1 Recombinant antibody (83456-6-RR)

    IF Staining of Caco-2 using 83456-6-RR

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using PMS1 antibody (83456-6-RR, Clone: 240408D9 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using PMS1 Recombinant antibody (83456-6-RR)

    FC experiment of HeLa using 83456-6-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug PMS1 Recombinant antibody (83456-6-RR, Clone:240408D9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83456-6-RR

    Affinity and Kinetic Characterization of 83456-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83456-6-RR against Human PMS1 were performed. The affinity constant is 0.367 nM.

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, HEK-293T cells, K-562 cells, Jurkat cells, MOLT-4 cells, HL-60 cells
Positive IF/ICC detected inCaco-2 cells, HeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83456-6-RR is PMS1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PMS1 fusion protein Ag28132 相同性解析による交差性が予測される生物種
Full Name PMS1 postmeiotic segregation increased 1 (S. cerevisiae)
Observed molecular weight 106 kDa
GenBank accession numberBC096331
Gene symbol PMS1
Gene ID (NCBI) 5378
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PMS1 Homolog 1 is a member of the DNA mismatch repair enzymes (MMREs) which is to eliminate the mismatch of insertions and deletions as a consequence of DNA polymerase errors at DNA synthesis in eukaryotes. PMS1 and MLH1 could form a heterodimer MutLβ that belongs to MMREs (PMID:25619773). In the MMR system, the MutS complex recognizes the DNA mispairs firstly, and then the Mlh1-Pms1 and other associated proteins such as PCNA, RFC, ExoI were recruited, inducing Exonuclease 1 (Exo1)-dependent and -independent MMR pathways (PMID:24981171). Mutations in this gene cause hereditary nonpolyposis colorectal cancer(HNPCC), which is also known as Lynch syndrome. It was reported that PMS1 is a widely expressed protein and located in the nucleus. Alternative splicing allows for 4 isoforms of PMS1 protein.

Protocols

Product Specific Protocols
WB protocol for PMS1 antibody 83456-6-RRDownload protocol
IF protocol for PMS1 antibody 83456-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols