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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PNPT1 Monoclonal antibody (68309-1-Ig)

    WB analysis using 68309-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68309-1-Ig (PNPT1 antibody) at dilution of 1:10000 and incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PNPT1 Monoclonal antibody (68309-1-Ig)

    IF Staining of HeLa using 68309-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PNPT1 antibody (68309-1-Ig, Clone: 3F2A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

PNPT1 Monoclonal antibody

PNPT1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68309-1-Ig
Clone No.3F2A1

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IF/ICC, ELISA

Synonyms

3 5 RNA exonuclease OLD35, DKFZp762K1914, old 35, OLD35, PNPASE, PNPase 1, PNPase old 35, PNPT1, Polynucleotide phosphorylase 1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, LNCap cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68309-1-Ig targets PNPT1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PNPT1 fusion protein Ag6290 相同性解析による交差性が予測される生物種
Full Name polyribonucleotide nucleotidyltransferase 1
Calculated molecular weight 86 kDa
Observed molecular weight 86 kDa
GenBank accession numberBC053660
Gene Symbol PNPT1
Gene ID (NCBI) 87178
RRIDAB_2935388
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8TCS8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PNPT1 (polynucleotide phosphorylase1; also known as PNPASE) localizes in the mitochondrial intermembrane and regulates RNA import into mitochondria (20691904). PNPT1 is also involved in mRNA degradation. As a type I IFN-inducible gene, PNPT1 plays an essential role in mediating IFN-mediated inflammatory processes (17804700). Recently mutation in PNPT1 has been reported to cause hereditary hearing loss (23084290). This antibody detected the endogenous PNPT1 around 80 kDa in mouse brain.

Protocols

Product Specific Protocols
WB protocol for PNPT1 antibody 68309-1-IgDownload protocol
IF protocol for PNPT1 antibody 68309-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols