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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CoraLite® Plus 488-conjugated PNPT1 Monoclonal ant (CL488-68309)

    IF Staining of A431 using CL488-68309

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 PNPT1 antibody (CL488-68309, Clone: 3F2A1 ) at dilution of 1:100, CL594-Phalloidin (red).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

CoraLite® Plus 488-conjugated PNPT1 Monoclonal antibody

PNPT1 Monoclonal Antibody for IF/ICC
Cat No. CL488-68309
Clone No.3F2A1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68309 targets PNPT1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PNPT1 fusion protein Ag6290 相同性解析による交差性が予測される生物種
Full Name polyribonucleotide nucleotidyltransferase 1
Calculated molecular weight 86 kDa
Observed molecular weight 86 kDa
GenBank accession numberBC053660
Gene Symbol PNPT1
Gene ID (NCBI) 87178
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8TCS8
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PNPT1 (polynucleotide phosphorylase1; also known as PNPASE) localizes in the mitochondrial intermembrane and regulates RNA import into mitochondria (20691904). PNPT1 is also involved in mRNA degradation. As a type I IFN-inducible gene, PNPT1 plays an essential role in mediating IFN-mediated inflammatory processes (17804700). Recently mutation in PNPT1 has been reported to cause hereditary hearing loss (23084290). This antibody detected the endogenous PNPT1 around 80 kDa in mouse brain.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 PNPT1 antibody CL488-68309Download protocol
Standard Protocols
Click here to view our Standard Protocols