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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated POFUT1 Polyclonal antibody

POFUT1 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-14929

Synonyms

O FucT 1, O Fuc T, KIAA0180, GDP-fucose protein O-fucosyltransferase 1, FUT12

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using CoraLite®594-conjugated POFUT1 Polyclonal antibody (CL594-14929)

    IF Staining of HUVEC using CL594-14929

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using CoraLite®594 POFUT1 antibody (CL594-14929) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated POFUT1 Polyclonal antibody (CL594-14929)

    IF Staining of HepG2 using CL594-14929

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 POFUT1 antibody (CL594-14929) at dilution of 1:200.

  • Flow cytometry (FC) experiment of A431 cells using CoraLite®594-conjugated POFUT1 Polyclonal antibody (CL594-14929)

    FC experiment of A431 using CL594-14929

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human POFUT1 (CL594-14929) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHUVEC cells, HepG2 cells
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-14929 targets POFUT1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POFUT1 fusion protein Ag6710 相同性解析による交差性が予測される生物種
Full Name protein O-fucosyltransferase 1
Calculated molecular weight 44 kDa
Observed molecular weight 44 kDa
GenBank accession numberBC000582
Gene symbol POFUT1
Gene ID (NCBI) 23509
RRIDAB_2919815
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

POFUT1 (protein O-fucosyltransferase 1), also known as GDP-fucose protein O-fucosyltransferase, is an enzyme that catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in EGF domains and plays a crucial role in Notch signaling.

Protocols

Product Specific Protocols
IF protocol for CL594 POFUT1 antibody CL594-14929Download protocol
FC protocol for CL594 POFUT1 antibody CL594-14929Download protocol
Standard Protocols
Click here to view our Standard Protocols