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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PON3 Polyclonal antibody (31793-1-AP)

    WB analysis using 31793-1-AP (same clone as 31793-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31793-1-AP (PON3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31793-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using PON3 Polyclonal antibody (31793-1-AP)

    IHC staining of mouse liver using 31793-1-AP (same clone as 31793-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 31793-1-AP (PON3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31793-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using PON3 Polyclonal antibody (31793-1-AP)

    IHC staining of human liver using 31793-1-AP (same clone as 31793-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 31793-1-AP (PON3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31793-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using PON3 Polyclonal antibody (31793-1-AP)

    IHC staining of human liver using 31793-1-AP (same clone as 31793-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 31793-1-AP (PON3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31793-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat liver tissue using PON3 Polyclonal antibody (31793-1-AP)

    IHC staining of rat liver using 31793-1-AP (same clone as 31793-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 31793-1-AP (PON3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31793-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat liver tissue using PON3 Polyclonal antibody (31793-1-AP)

    IHC staining of rat liver using 31793-1-AP (same clone as 31793-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 31793-1-AP (PON3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31793-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using PON3 Polyclonal antibody (31793-1-AP)

    IHC staining of mouse liver using 31793-1-AP (same clone as 31793-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 31793-1-AP (PON3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31793-1-PBS in a different storage buffer formulation.

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PON3 Polyclonal antibody, PBS Only

PON3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 31793-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Paraoxonase 3 , Paraoxanase-3, EC:3.1.8.1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

31793-1-PBS targets PON3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PON3 fusion protein Ag36602 相同性解析による交差性が予測される生物種
Full Name paraoxonase 3
Calculated molecular weightNM_000940.2 40kDa，354aa
Observed molecular weight40 kDa
GenBank accession numberNM_000940.2
Gene Symbol PON3
Gene ID (NCBI) 5446
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ15166
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

PON3, a member of the paraoxonase family, is expressed primarily in the liver and encodes a protein that is secreted into the bloodstream and binds to high-density lipoprotein (HDL). It also rapidly hydrolyzes lactones and inhibits the oxidation of low-density lipoprotein (LDL), thereby slowing the onset and progression of atherosclerosis. In addition, PON3 is closely associated with hepatocellular carcinoma (HCC), and is a potential therapeutic target for HCC by inducing cell cycle arrest and further inhibiting HCC cell proliferation.