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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

POT1 Recombinant antibody, PBS Only (Detector)

POT1 Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240688E5

Cat no : 83732-2-PBS

Synonyms

POT1-like telomere end-binding protein, hPot1, 240688E5

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using POT1 Recombinant antibody (83732-2-RR)

    IF Staining of HeLa using 83732-2-RR (same clone as 83732-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using POT1 antibody (83732-2-RR, Clone: 240688E5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83732-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using POT1 Recombinant antibody (83732-2-RR)

    IF Staining of HeLa using 83732-2-RR (same clone as 83732-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using POT1 antibody (83732-2-RR, Clone: 240688E5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83732-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U2OS cells using POT1 Recombinant antibody (83732-2-RR)

    FC experiment of U2OS using 83732-2-RR (same clone as 83732-2-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug POT1 Recombinant antibody (83732-2-RR, Clone:240688E5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83732-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of SH-SY5Y cells using POT1 Recombinant antibody (83732-2-RR)

    FC experiment of SH-SY5Y using 83732-2-RR (same clone as 83732-2-PBS)

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug POT1 Recombinant antibody (83732-2-RR, Clone:240688E5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83732-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00711-1

    Cytometric bead array standard curve of MP00711-1

    Cytometric bead array standard curve of MP00711-1, POT1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83732-4-PBS. Detection antibody: 83732-2-PBS. Standard: Ag0875. Range: 0.78-100 ng/mL.

  • Affinity and Kinetic Characterization of 83732-2-RR

    Affinity and Kinetic Characterization of 83732-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83732-2-RR against Human POT1 were performed. The affinity constant is 2.57 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83732-2-PBS targets POT1 as part of a matched antibody pair:

MP00711-1: 83732-4-PBS capture and 83732-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen POT1 fusion protein Ag0875 相同性解析による交差性が予測される生物種
Full Name POT1 protection of telomeres 1 homolog (S. pombe)
Calculated molecular weight 71 kDa
GenBank accession numberBC002923
Gene symbol POT1
Gene ID (NCBI) 25913
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Protection of telomeres 1(POT1) is a conserved protein that binds the G-rich stand of its own telomeric repeat sequence, with a role in protecting chromosome ends. It's housekeeping gene that is required to ensure the integrity of chromosome ends in all cells. It's a component of the telomerase ribonucleoprotein(RNP) complex that is esential for the replication of chromosome termini. It consists the TRF1 compex that is involved in the regulation of telomere length by cis-inhibition of telomerase. We have discovered that POT1 exists in at least three consistently occurring forms; 90, 70 and 45kDa. The unexpected molecular weights of POT1 seem to be associated with SUMO1 and ubiquitin conjugation; the latter occurring at a double lysine residue at 289-KK-290 (.PMID: 24054699)