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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using POT1 Recombinant antibody (83732-2-RR)

    IF Staining of HeLa using 83732-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using POT1 antibody (83732-2-RR, Clone: 240688E5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using POT1 Recombinant antibody (83732-2-RR)

    IF Staining of HeLa using 83732-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using POT1 antibody (83732-2-RR, Clone: 240688E5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of U2OS cells using POT1 Recombinant antibody (83732-2-RR)

    FC experiment of U2OS using 83732-2-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug POT1 Recombinant antibody (83732-2-RR, Clone:240688E5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Flow cytometry (FC) experiment of SH-SY5Y cells using POT1 Recombinant antibody (83732-2-RR)

    FC experiment of SH-SY5Y using 83732-2-RR

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug POT1 Recombinant antibody (83732-2-RR, Clone:240688E5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83732-2-RR

    Affinity and Kinetic Characterization of 83732-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83732-2-RR against Human POT1 were performed. The affinity constant is 2.57 nM.

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POT1 Recombinant antibody

POT1 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83732-2-RR
Clone No.240688E5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

POT1-like telomere end-binding protein, hPot1, 240688E5

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inU2OS cells, SH-SY5Y cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83732-2-RR targets POT1 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen POT1 fusion protein Ag0875 相同性解析による交差性が予測される生物種
Full Name POT1 protection of telomeres 1 homolog (S. pombe)
Calculated molecular weight 71 kDa
GenBank accession numberBC002923
Gene Symbol POT1
Gene ID (NCBI) 25913
RRIDAB_3671332
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NUX5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protection of telomeres 1(POT1) is a conserved protein that binds the G-rich stand of its own telomeric repeat sequence, with a role in protecting chromosome ends. It's housekeeping gene that is required to ensure the integrity of chromosome ends in all cells. It's a component of the telomerase ribonucleoprotein(RNP) complex that is esential for the replication of chromosome termini. It consists the TRF1 compex that is involved in the regulation of telomere length by cis-inhibition of telomerase. We have discovered that POT1 exists in at least three consistently occurring forms; 90, 70 and 45kDa. The unexpected molecular weights of POT1 seem to be associated with SUMO1 and ubiquitin conjugation; the latter occurring at a double lysine residue at 289-KK-290 (.PMID: 24054699)

Protocols

Product Specific Protocols
IF protocol for POT1 antibody 83732-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols