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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A549 cells using CoraLite® Plus 488-conjugated POTEA-Specific Recom (CL488-84298)

    IF Staining of A549 using CL488-84298

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite® Plus 488 POTEA-Specific antibody (CL488-84298, Clone: 241187F7 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated POTEA-Specific Recom (CL488-84298)

    FC experiment of HeLa using CL488-84298

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Coralite® Plus 488 POTEA-Specific Recombinant Antibody (CL488-84298, Clone:241187F7)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

CoraLite® Plus 488-conjugated POTEA-Specific Recombinant antibody

POTEA-Specific Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra)
Cat No. CL488-84298
Clone No.241187F7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

POTEA, POTE8, POTE 8, ANKRD26-like family A member 1, ANKRD26 like family A member 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-84298 targets POTEA-Specific in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name POTE ankyrin domain family, member A
Calculated molecular weight 56 kDa
GenBank accession numberNM_001002920
Gene Symbol POTEA
Gene ID (NCBI) 340441
RRIDAB_3673352
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ6S8J7
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

POTEA, also named A26A1 and POTE8, belongs to the POTE family. POTE has an important signaling function in the reproductive system.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 POTEA-Specific antibody CL488-84298Download protocol
Standard Protocols
Click here to view our Standard Protocols