POU2AF1 Monoclonal antibody, PBS Only (Capture/Detector)
POU2AF1 Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
WB, Cytometric bead array, Indirect ELISA
Conjugate
Unconjugated
CloneNo.
3C5A7
Cat no : 66659-1-PBS
Synonyms
Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
66659-1-PBS targets POU2AF1 as part of a matched antibody pair:
MP51058-1: 66659-2-PBS capture and 66659-1-PBS detection (validated in Cytometric bead array)
MP51058-2: 66659-1-PBS capture and 66659-3-PBS detection (validated in Cytometric bead array)
Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | POU2AF1 fusion protein Ag23613 相同性解析による交差性が予測される生物種 |
| Full Name | POU class 2 associating factor 1 |
| Calculated molecular weight | 256 aa, 27 kDa |
| Observed molecular weight | 35 kDa |
| GenBank accession number | BC032549 |
| Gene symbol | POU2AF1 |
| Gene ID (NCBI) | 5450 |
| RRID | AB_2882016 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS Only |
| Storage Conditions | Store at -80°C. |
Background Information
POU2AF1, also named as BOB-1 or OCA-B, is a 256 amino acid protein, which belongs to the POU2AF1 family. POU2AF1 is expressed in B-cell specific. POU2AF1 as a transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.The predicted size of this protein is 27 kDa. Studies have reported that the protein has a multi-band size of 34-35 kDa through siRNA interference experiments (PMID: 17621271). This result is the same as our experiments.
