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POU2AF1 Monoclonal antibody

POU2AF1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66659-1-Ig
Clone No.3C5A7

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

OCAB, OBF1, BOB-1, BOB1, B-cell-specific coactivator OBF-1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration:  150 μL, 1700 ug/ml
20 μL, 1700 ug/ml
150 μL, 1700 ug/ml

¥67,000/ 150 μL

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inRaji cells, Ramos cells, Daudi cells
Positive IHC detected inHuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:850-1:3400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66659-1-Ig targets POU2AF1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen POU2AF1 fusion protein Ag23613 相同性解析による交差性が予測される生物種
Full Name POU class 2 associating factor 1
Calculated molecular weight 256 aa, 27 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC032549
Gene Symbol POU2AF1
Gene ID (NCBI) 5450
ENSEMBL Gene IDENSG00000110777
RRIDAB_2882016
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16633
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

POU2AF1, also named as BOB-1 or OCA-B, is a 256 amino acid protein, which belongs to the POU2AF1 family. POU2AF1 is expressed in B-cell specific. POU2AF1 as a transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.The predicted size of this protein is 27 kDa. Studies have reported that the protein has a multi-band size of 34-35 kDa through siRNA interference experiments (PMID: 17621271). This result is the same as our experiments.

Protocols

Product Specific Protocols
WB protocol for POU2AF1 antibody 66659-1-IgDownload protocol
IHC protocol for POU2AF1 antibody 66659-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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