Validation Data Gallery
Tested Applications
| Positive WB detected in | Raji cells, Ramos cells, Daudi cells |
| Positive IHC detected in | Human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:850-1:3400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66659-1-Ig targets POU2AF1 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | POU2AF1 fusion protein Ag23613 相同性解析による交差性が予測される生物種 |
| Full Name | POU class 2 associating factor 1 |
| Calculated molecular weight | 256 aa, 27 kDa |
| Observed molecular weight | 35 kDa |
| GenBank accession number | BC032549 |
| Gene Symbol | POU2AF1 |
| Gene ID (NCBI) | 5450 |
| ENSEMBL Gene ID | ENSG00000110777 |
| RRID | AB_2882016 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q16633 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
POU2AF1, also named as BOB-1 or OCA-B, is a 256 amino acid protein, which belongs to the POU2AF1 family. POU2AF1 is expressed in B-cell specific. POU2AF1 as a transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.The predicted size of this protein is 27 kDa. Studies have reported that the protein has a multi-band size of 34-35 kDa through siRNA interference experiments (PMID: 17621271). This result is the same as our experiments.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for POU2AF1 antibody 66659-1-Ig | Download protocol |
| IHC protocol for POU2AF1 antibody 66659-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |