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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated POU2AF1 Monoclonal antibody

POU2AF1 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3C5A7

Cat no : CL488-66659

Synonyms

OCAB, OBF1, BOB-1, BOB1, B-cell-specific coactivator OBF-1

Validation Data Gallery

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  • Flow cytometry (FC) experiment of Ramos cells using CoraLite® Plus 488-conjugated POU2AF1 Monoclonal a (CL488-66659)

    FC experiment of Ramos using CL488-66659

    1x10^6 Ramos cells were intracellularly stained with 0.2 ug Coralite® Plus 488 Pou2af1 Monoclonal Antibody (CL488-66659, Clone:3C5A7)(red), or 0.2 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (1F8D3) (CL488-66360-1, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive FC (Intra) detected inRamos cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66659 targets POU2AF1 in FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen POU2AF1 fusion protein Ag23613 相同性解析による交差性が予測される生物種
Full Name POU class 2 associating factor 1
Calculated molecular weight 256 aa, 27 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC032549
Gene symbol POU2AF1
Gene ID (NCBI) 5450
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

POU2AF1, also named as BOB-1 or OCA-B, is a 256 amino acid protein, which belongs to the POU2AF1 family. POU2AF1 is expressed in B-cell specific. POU2AF1 as a transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.The predicted size of this protein is 27 kDa. Studies have reported that the protein has a multi-band size of 34-35 kDa through siRNA interference experiments (PMID: 17621271). This result is the same as our experiments.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 POU2AF1 antibody CL488-66659Download protocol
Standard Protocols
Click here to view our Standard Protocols