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POU4F3 Recombinant antibody

POU4F3 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA
Cat No. 82980-1-RR
Clone No.230345F1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

BRN3C, Brn 3C, Brain-specific homeobox/POU domain protein 3C, Brain-3C, Brain 3C

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inNeuro-2a cells, mouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat brain tissue
Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inU-251 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:175-1:700
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82980-1-RR targets POU4F3 in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen POU4F3 fusion protein Ag16004 相同性解析による交差性が予測される生物種
Full Name POU class 4 homeobox 3
Calculated molecular weight 338 aa, 37 kDa
Observed molecular weight 33-40 kDa
GenBank accession numberBC104923
Gene Symbol POU4F3
Gene ID (NCBI) 5459
RRIDAB_3670729
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15319
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for POU4F3 antibody 82980-1-RRDownload protocol
IHC protocol for POU4F3 antibody 82980-1-RRDownload protocol
IF protocol for POU4F3 antibody 82980-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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