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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated PPA1 Monoclonal antibody

PPA1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D11F4

Cat no : CL488-68178

Synonyms

PP1, PP, IOPPP, Inorganic pyrophosphatase, EC:3.6.1.1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated PPA1 Monoclonal anti (CL488-68178)

    IF Staining of HeLa using CL488-68178

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 PPA1 antibody (CL488-68178, Clone: 1D11F4 ) at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated PPA1 Monoclonal anti (CL488-68178)

    IF Staining of HeLa using CL488-68178

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 PPA1 antibody (CL488-68178, Clone: 1D11F4 ) at dilution of 1:100.

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68178 targets PPA1 in IF/ICC applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PPA1 fusion protein Ag7049 相同性解析による交差性が予測される生物種
Full Name pyrophosphatase (inorganic) 1
Calculated molecular weight 33 kDa
Observed molecular weight33 kDa
GenBank accession numberBC001022
Gene symbol PPA1
Gene ID (NCBI) 5464
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PPA1(Inorganic pyrophosphatase) is also named as IOPPP, PP and PPase. It maintains the thermodynamic favorability of these reactions by catalyzing the hydrolysis of pyrophosphates into organic phosphates, which are then exported across the cell membrane(PMID:16300924).The erythrocyte inorganic pyrophosphatase molecule would appear to be a dimer(PMID:4130389).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 PPA1 antibody CL488-68178Download protocol
Standard Protocols
Click here to view our Standard Protocols