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  • Featured Product
  • KD/KO Validated

PPAN Polyclonal antibody, PBS Only

PPAN Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11006-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPAN Polyclonal antibody (11006-1-AP)

    WB analysis using 11006-1-AP (same clone as 11006-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11006-1-AP (PPAN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11006-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using PPAN Polyclonal antibody (11006-1-AP)

    WB analysis of HEK-293 using 11006-1-AP (same clone as 11006-1-PBS)

    WB result of PPAN antibody (11006-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPAN transfected HEK-293 cells. This data was developed using the same antibody clone with 11006-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using PPAN Polyclonal antibody (11006-1-AP)

    IHC staining of human colon using 11006-1-AP (same clone as 11006-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11006-1-AP (PPAN antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11006-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11006-1-PBS targets PPAN in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPAN fusion protein Ag1419 相同性解析による交差性が予測される生物種
Full Name peter pan homolog (Drosophila)
Calculated molecular weight 53 kDa
Observed molecular weight 55-58 kDa
GenBank accession numberBC009833
Gene symbol PPAN
Gene ID (NCBI) 56342
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PPAN (or SSF, SSF1 or SSF2) gene is widely expressed, and encodes peter pan homolog protein which may be localized in nucleus. Two isoforms are produced through alternative splicing. So far the characterization of this protein is largely unknown, multiple phosphorylation sites has been revealed based on high-throughput phosphorylation analysis. This antibody is raised against the C-terminal of huaman PPAN and recognize bands around 55 kDa.