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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PPCS Polyclonal antibody (18001-1-AP)

    WB analysis of HeLa using 18001-1-AP (same clone as 18001-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 18001-1-AP (PPCS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18001-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PPCS Polyclonal antibody (18001-1-AP)

    IHC staining of human kidney using 18001-1-AP (same clone as 18001-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 18001-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PPCS Polyclonal antibody (18001-1-AP)

    IHC staining of human kidney using 18001-1-AP (same clone as 18001-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 18001-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using PPCS Polyclonal antibody (18001-1-AP)

    IHC staining of human placenta using 18001-1-AP (same clone as 18001-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 18001-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using PPCS Polyclonal antibody (18001-1-AP)

    IHC staining of human placenta using 18001-1-AP (same clone as 18001-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 18001-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using PPCS Polyclonal antibody (18001-1-AP)

    IHC staining of human testis using 18001-1-AP (same clone as 18001-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 18001-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using PPCS Polyclonal antibody (18001-1-AP)

    IHC staining of human testis using 18001-1-AP (same clone as 18001-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 18001-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using PPCS Polyclonal antibody (18001-1-AP)

    IHC staining of human spleen using 18001-1-AP (same clone as 18001-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 18001-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using PPCS Polyclonal antibody (18001-1-AP)

    IHC staining of human spleen using 18001-1-AP (same clone as 18001-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 18001-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using PPCS Polyclonal antibody (18001-1-AP)

    IHC staining of human lung using 18001-1-AP (same clone as 18001-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 18001-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using PPCS Polyclonal antibody (18001-1-AP)

    IHC staining of human lung using 18001-1-AP (same clone as 18001-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 18001-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tissue using PPCS Polyclonal antibody (18001-1-AP)

    IHC staining of human ovary using 18001-1-AP (same clone as 18001-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 18001-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tissue using PPCS Polyclonal antibody (18001-1-AP)

    IHC staining of human ovary using 18001-1-AP (same clone as 18001-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 18001-1-PBS in a different storage buffer formulation.

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PPCS Polyclonal antibody, PBS Only

PPCS Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 18001-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18001-1-PBS targets PPCS in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPCS fusion protein Ag12491 相同性解析による交差性が予測される生物種
Full Name phosphopantothenoylcysteine synthetase
Calculated molecular weight 311 aa, 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC106064
Gene Symbol PPCS
Gene ID (NCBI) 79717
RRIDAB_10548725
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HAB8
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

PPCS(Phosphopantothenate--cysteine ligase) is also named as COAB and belongs to the PPC synthetase family. It catalyzes the first step in the biosynthesis of coenzyme A from vitamin B5, where cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine. It can be exsit as a homodimer(PMID:12906824).