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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated PPP1CB-Specific Polyclonal antibody

PPP1CB-Specific Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-55136

Synonyms

PPP1CB, PPP1CD, PP-1B, PP1 Beta, PP 1B

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CoraLite® Plus 488-conjugated PPP1CB-Specific Poly (CL488-55136)

    IF Staining of U2OS using CL488-55136

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite® Plus 488 PPP1CB-Specific antibody (CL488-55136) at dilution of 1:200, CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of U2OS cells using CoraLite® Plus 488-conjugated PPP1CB-Specific Poly (CL488-55136)

    FC experiment of U2OS using CL488-55136

    1x10^6 U2OS cells were intracellularly stained with 0.8 ug Coralite® Plus 488 PPP1CB-Specific Polyclonal Antibody (CL488-55136)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inU2OS cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-55136 targets PPP1CB-Specific in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 1, catalytic subunit, beta isoform
Calculated molecular weight 37 kDa
Observed molecular weight 33-40 kDa
GenBank accession numberNM_002709
Gene symbol PPP1CB
Gene ID (NCBI) 5500
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PPP1CB, also named as PP-1B, belongs to the PPP phosphatase family and PP-1 subfamily. Protein phosphatase (PP1) is essential for cell division, it participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. PPP1CB is involved in regulation of ionic conductances and long-term synaptic plasticity. This antibody is specific to PPP1CB.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 PPP1CB-Specific antibody CL488-55136Download protocol
FC protocol for CL Plus 488 PPP1CB-Specific antibody CL488-55136Download protocol
Standard Protocols
Click here to view our Standard Protocols