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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPP1R13B Polyclonal antibody (29804-1-AP)

    WB analysis using 29804-1-AP (same clone as 29804-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29804-1-AP (PPP1R13B antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29804-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PPP1R13B Polyclonal antibody (29804-1-AP)

    IHC staining of human lung cancer using 29804-1-AP (same clone as 29804-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29804-1-AP (PPP1R13B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29804-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PPP1R13B Polyclonal antibody (29804-1-AP)

    IHC staining of mouse testis using 29804-1-AP (same clone as 29804-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29804-1-AP (PPP1R13B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29804-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PPP1R13B Polyclonal antibody (29804-1-AP)

    IHC staining of human lung cancer using 29804-1-AP (same clone as 29804-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29804-1-AP (PPP1R13B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29804-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PPP1R13B Polyclonal antibody (29804-1-AP)

    IHC staining of mouse testis using 29804-1-AP (same clone as 29804-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29804-1-AP (PPP1R13B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29804-1-PBS in a different storage buffer formulation.

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PPP1R13B Polyclonal antibody, PBS Only

PPP1R13B Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 29804-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29804-1-PBS targets PPP1R13B in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPP1R13B fusion protein Ag30788 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 1, regulatory (inhibitor) subunit 13B
Calculated molecular weight 1090 aa, 120 kDa
Observed molecular weight120 kDa
GenBank accession numberBC136527
Gene Symbol PPP1R13B
Gene ID (NCBI) 23368
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96KQ4
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

PPP1R13B(Protein phosphatase 1 regulatory subunit 13B), also named ASPP1 and KIAA0771, is a member of the apoptosis-stimulating proteins of the p53 family (ASPPs)(PMID: 30908929). PPP1R13B contains four ankyrin repeats and an SH3 domain involved in protein-protein interactions. PPP1R13 promotes DNA binding and transactivation of p53-family proteins on the promoters of proapoptotic genes. Expression of this gene is regulated by the E2F transcription factor.