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  • Featured Product
  • KD/KO Validated

PPP1R15B Recombinant antibody, PBS Only

PPP1R15B Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240052G5

Cat no : 83016-6-PBS

Synonyms

PPP1R15B;CReP, FLJ14744, CREP, 240052G5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPP1R15B Recombinant antibody (83016-6-RR)

    WB analysis using 83016-6-RR (same clone as 83016-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83016-6-RR (PPP1R15B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83016-6-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SGC-7901 cells using PPP1R15B Recombinant antibody (83016-6-RR)

    WB analysis of SGC-7901 using 83016-6-RR (same clone as 83016-6-PBS)

    WB result of PPP1R15B antibody (83016-6-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPP1R15B transfected SGC-7901 cells. This data was developed using the same antibody clone with 83016-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PPP1R15B Recombinant antibody (83016-6-RR)

    IF Staining of HepG2 using 83016-6-RR (same clone as 83016-6-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PPP1R15B antibody (83016-6-RR, Clone: 240052G5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83016-6-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U2OS cells using PPP1R15B Recombinant antibody (83016-6-RR)

    FC experiment of U2OS using 83016-6-RR (same clone as 83016-6-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug PPP1R15B Recombinant antibody (83016-6-RR, Clone:240052G5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83016-6-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83016-6-PBS is PPP1R15B Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PPP1R15B fusion protein Ag6213 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 1, regulatory (inhibitor) subunit 15B
Calculated molecular weight 79 kDa
GenBank accession numberBC065280
Gene symbol PPP1R15B
Gene ID (NCBI) 84919
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.