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  • Featured Product
  • KD/KO Validated

PPP1R15B Recombinant antibody

PPP1R15B Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240052G5

Cat no : 83016-6-RR

Synonyms

FLJ14744, CREP, 240052G5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPP1R15B Recombinant antibody (83016-6-RR)

    WB analysis using 83016-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83016-6-RR (PPP1R15B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SGC-7901 cells using PPP1R15B Recombinant antibody (83016-6-RR)

    WB analysis of SGC-7901 using 83016-6-RR

    WB result of PPP1R15B antibody (83016-6-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPP1R15B transfected SGC-7901 cells.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PPP1R15B Recombinant antibody (83016-6-RR)

    IF Staining of HepG2 using 83016-6-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PPP1R15B antibody (83016-6-RR, Clone: 240052G5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of U2OS cells using PPP1R15B Recombinant antibody (83016-6-RR)

    FC experiment of U2OS using 83016-6-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug PPP1R15B Recombinant antibody (83016-6-RR, Clone:240052G5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inSGC-7901 cells, A2780 cells, MDA-MB-231 cells, SKOV-3 cells
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83016-6-RR is PPP1R15B Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PPP1R15B fusion protein Ag6213 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 1, regulatory (inhibitor) subunit 15B
Calculated molecular weight 79 kDa
Observed molecular weight100-110 kDa
GenBank accession numberBC065280
Gene symbol PPP1R15B
Gene ID (NCBI) 84919
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein phosphatase 1 regulatory subunit 15B(PPP1R15B) is a member of PPP1R15 family, it forms a complex with protein phosphatase1(PP1) and NCK1/2, maintaining low levels of EIF2S1 phosphorylation in unstressed cells, thus is also named constitutive repressor of eIF2alpha phosphorylation (CReP). PPP1R15B is assoicated in endoplasmic reticulum (ER) stress response resulting to a role in translation regulation. Knock-down of PPP1R15B strongly protected mammalian cells against oxidative stress, peroxynitrite stress, and more modestly against accumulation of malfolded proteins in the ER. Recently, PPP1R15B was found to associate with cell membranes and regulate membrane traffic in a PP1c-independent manner, suggesting a novel link between translation and traffic. MW of PPP1R15B is 80 kDa. Observed MW of PPP1R15B is from 100-110 kDa maybe due to phosphorylation (PMID: 28492545).

Protocols

Product Specific Protocols
WB protocol for PPP1R15B antibody 83016-6-RRDownload protocol
IF protocol for PPP1R15B antibody 83016-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols