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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated PPP1R15B Recombinant antibody

PPP1R15B Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

240052G5

Cat no : CL488-83016-6

Synonyms

FLJ14744, CREP, 240052G5

Validation Data Gallery

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  • Flow cytometry (FC) experiment of U-2 OS using CoraLite® Plus 488-conjugated PPP1R15B Recombinant (CL488-83016-6)

    FC experiment of U-2 OS using CL488-83016-6

    1x10^6 U-2 OS were intracellularly stained with 0.4 ug Coralite® Plus 488 Ppp1r15b Recombinant Antibody (CL488-83016-6, Clone:240052G5), and 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated PPP1R15B Recombinant (CL488-83016-6)

    FC experiment of HepG2 using CL488-83016-6

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug Coralite® Plus 488 Ppp1r15b Recombinant Antibody (CL488-83016-6, Clone:240052G5)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive FC (Intra) detected inU-2 OS, HepG2 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-83016-6 is PPP1R15B Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PPP1R15B fusion protein Ag6213 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 1, regulatory (inhibitor) subunit 15B
Calculated molecular weight 79 kDa
Observed molecular weight100-110 kDa
GenBank accession numberBC065280
Gene symbol PPP1R15B
Gene ID (NCBI) 84919
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein phosphatase 1 regulatory subunit 15B(PPP1R15B) is a member of PPP1R15 family, it forms a complex with protein phosphatase1(PP1) and NCK1/2, maintaining low levels of EIF2S1 phosphorylation in unstressed cells, thus is also named constitutive repressor of eIF2alpha phosphorylation (CReP). PPP1R15B is assoicated in endoplasmic reticulum (ER) stress response resulting to a role in translation regulation. Knock-down of PPP1R15B strongly protected mammalian cells against oxidative stress, peroxynitrite stress, and more modestly against accumulation of malfolded proteins in the ER. Recently, PPP1R15B was found to associate with cell membranes and regulate membrane traffic in a PP1c-independent manner, suggesting a novel link between translation and traffic. MW of PPP1R15B is 80 kDa. Observed MW of PPP1R15B is from 100-110 kDa maybe due to phosphorylation (PMID: 28492545).

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 PPP1R15B antibody CL488-83016-6Download protocol
Standard Protocols
Click here to view our Standard Protocols