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  • Featured Product
  • KD/KO Validated

PPP2CA Polyclonal antibody, PBS Only

PPP2CA Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13482-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using PPP2CA Polyclonal antibody (13482-1-AP)

    WB analysis of Jurkat using 13482-1-AP (same clone as 13482-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13482-1-AP (PPP2CA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13482-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PPP2CA Polyclonal antibody (13482-1-AP)

    WB analysis using 13482-1-AP (same clone as 13482-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13482-1-AP (PPP2CA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13482-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human liver tissue using PPP2CA Polyclonal antibody (13482-1-AP)

    WB analysis of human liver using 13482-1-AP (same clone as 13482-1-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 13482-1-AP (PPP2CA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13482-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using PPP2CA Polyclonal antibody (13482-1-AP)

    WB analysis of MCF-7 using 13482-1-AP (same clone as 13482-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 13482-1-AP (PPP2CA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13482-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293T cells using PPP2CA Polyclonal antibody (13482-1-AP)

    WB analysis of HEK-293T using 13482-1-AP (same clone as 13482-1-PBS)

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 13482-1-AP (PPP2CA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13482-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Jurkat cells using PPP2CA Polyclonal antibody (13482-1-AP)

    IP experiment of Jurkat using 13482-1-AP (same clone as 13482-1-PBS)

    IP result of anti-PPP2CA (IP:13482-1-AP, 4ug; Detection:13482-1-AP 1:500) with Jurkat cells lysate 2400ug. This data was developed using the same antibody clone with 13482-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using PPP2CA Polyclonal antibody (13482-1-AP)

    IHC staining of human liver using 13482-1-AP (same clone as 13482-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13482-1-AP (PPP2CA antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 13482-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using PPP2CA Polyclonal antibody (13482-1-AP)

    IHC staining of human liver using 13482-1-AP (same clone as 13482-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13482-1-AP (PPP2CA antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 13482-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using PPP2CA Polyclonal antibody (13482-1-AP)

    IF Staining of MCF-7 using 13482-1-AP (same clone as 13482-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PPP2CA antibody (13482-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 13482-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using PPP2CA Polyclonal antibody (13482-1-AP)

    FC experiment of MCF-7 using 13482-1-AP (same clone as 13482-1-PBS)

    1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human PPP2CA (13482-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 13482-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13482-1-PBS targets PPP2CA in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPP2CA fusion protein Ag4294 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform
Calculated molecular weight 309 aa, 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC031696
Gene symbol PPP2CA
Gene ID (NCBI) 5515
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PPP2CA, also named as RP-C and PP2A-alpha, belongs to the PPP phosphatase family and PP-1 subfamily. PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. PP2A activity was modestly decreased in AML patients harboring C-KIT/WT.PP2A is a target for C-KIT/TKD and implicated the potential efficacy of therapies targeting PP2A in such AML in future.