PPP2CA Monoclonal antibody
PPP2CA Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, rat and More (1)
Applications
WB, IHC, CoIP, ELISA
Conjugate
Unconjugated
CloneNo.
3A6H5
Cat no : 67809-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, U2OS cells, HeLa cells, HEK-293 cells, Jurkat cells, HSC-T6 cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
67809-1-Ig targets PPP2CA in WB, IHC, CoIP, ELISA applications and shows reactivity with Human, rat samples.
| Tested Reactivity | Human, rat |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | PPP2CA fusion protein Ag4294 相同性解析による交差性が予測される生物種 |
| Full Name | protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform |
| Calculated molecular weight | 309 aa, 36 kDa |
| Observed molecular weight | 33 kDa |
| GenBank accession number | BC031696 |
| Gene symbol | PPP2CA |
| Gene ID (NCBI) | 5515 |
| RRID | AB_2918572 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PPP2CA, also named as RP-C and PP2A-alpha, belongs to the PPP phosphatase family and PP-1 subfamily. PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. PP2A activity was modestly decreased in AML patients harboring C-KIT/WT.PP2A is a target for C-KIT/TKD and implicated the potential efficacy of therapies targeting PP2A in such AML in future.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PPP2CA antibody 67809-1-Ig | Download protocol |
| IHC protocol for PPP2CA antibody 67809-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Rep A Wnt-induced lncRNA-DGCR5 splicing switch drives tumor-promoting inflammation in esophageal squamous cell carcinoma | ||
Cell Signal METTL14-mediated N6-methyladenosine modification of TCP1 mRNA promotes acute myeloid leukemia progression |
