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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PPP2CA Monoclonal antibody, PBS Only

PPP2CA Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3A6H5

Cat no : 67809-1-PBS

Synonyms

PP2A alpha, PP2Ac, PP2CA, PPP2CA, Replication protein C, RP C

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPP2CA Monoclonal antibody (67809-1-Ig)

    WB analysis using 67809-1-Ig (same clone as 67809-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67809-1-Ig (PPP2CA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67809-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using PPP2CA Monoclonal antibody (67809-1-Ig)

    IHC staining of human liver using 67809-1-Ig (same clone as 67809-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67809-1-Ig (PPP2CA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67809-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using PPP2CA Monoclonal antibody (67809-1-Ig)

    IHC staining of human liver using 67809-1-Ig (same clone as 67809-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67809-1-Ig (PPP2CA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67809-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67809-1-PBS targets PPP2CA in WB, IHC, Indirect ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PPP2CA fusion protein Ag4294 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform
Calculated molecular weight 309 aa, 36 kDa
Observed molecular weight33 kDa
GenBank accession numberBC031696
Gene symbol PPP2CA
Gene ID (NCBI) 5515
RRIDAB_2918572
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PPP2CA, also named as RP-C and PP2A-alpha, belongs to the PPP phosphatase family and PP-1 subfamily. PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. PP2A activity was modestly decreased in AML patients harboring C-KIT/WT.PP2A is a target for C-KIT/TKD and implicated the potential efficacy of therapies targeting PP2A in such AML in future.