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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PPP3CA-specific Polyclonal antibody, PBS Only

PPP3CA-specific Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 55147-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using PPP3CA-specific Polyclonal antibody (55147-1-AP)

    WB analysis of mouse brain using 55147-1-AP (same clone as 55147-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 55147-1-AP (PPP3CA-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55147-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse pancreas tissue using PPP3CA-specific Polyclonal antibody (55147-1-AP)

    WB analysis of mouse pancreas using 55147-1-AP (same clone as 55147-1-PBS)

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 55147-1-AP (PPP3CA-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55147-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

55147-1-PBS targets PPP3CA-specific in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform
Calculated molecular weight 59 kDa
Observed molecular weight 59 kDa
GenBank accession numberNM_000944
Gene symbol PPP3CA
Gene ID (NCBI) 5530
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PPP3CA, also called calcineurin Aα, is the only serine/threonine protein phosphatase under the control of Ca2+/calmodulin, and plays a critical role in the coupling of Ca2+ signals to cellular responses. Ca2+ signaling plays a central role in hypertrophic growth of cardiac and skeletal muscle in response to mechanical load and a variety of signals. Therefore, PPP3CA plays an important role in muscle differentiation, especially in muscle fiber type conversion. It is also involved in osteoclast regulation, regulating bone formation through an effect on osteoblast differentiation. This antibody is specific to PPP3CA.