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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPP3CB Monoclonal antibody (66614-1-Ig)

    WB analysis using 66614-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66614-1-Ig (PPP3CB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using PPP3CB Monoclonal antibody (66614-1-Ig)

    WB analysis of rat brain using 66614-1-Ig

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66614-1-Ig (PPP3CB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using PPP3CB Monoclonal antibody (66614-1-Ig)

    WB analysis of mouse brain using 66614-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66614-1-Ig (PPP3CB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using PPP3CB Monoclonal antibody (66614-1-Ig)

    WB analysis of NIH/3T3 using 66614-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66614-1-Ig (PPP3CB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of fetal human brain tissue using PPP3CB Monoclonal antibody (66614-1-Ig)

    WB analysis of fetal human brain using 66614-1-Ig

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66614-1-Ig (PPP3CB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PPP3CB Monoclonal antibody (66614-1-Ig)

    IF Staining of HeLa using 66614-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PPP3CB antibody (66614-1-Ig, Clone: 3C1A3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using PPP3CB Monoclonal antibody (66614-1-Ig)

    IF Staining of U-251 using 66614-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using PPP3CB antibody (66614-1-Ig, Clone: 3C1A3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

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  • Featured Product
  • KD/KO Validated

PPP3CB Monoclonal antibody

PPP3CB Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 66614-1-Ig
Clone No.3C1A3
Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IF/ICC, ELISA

Synonyms

CALNA2, CAM-PRP catalytic subunit, CAM PRP catalytic subunit, CALNB, Calmodulin-dependent calcineurin A subunit beta isoform

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration:  150 μL, 1600 ug/ml
20 μL, 1600 ug/ml
150 μL, 1600 ug/ml

¥67,000/ 150 μL

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Tested Applications

Positive WB detected inpig brain tissue, NIH/3T3 cells, fetal human brain tissue, rat brain tissue, mouse brain tissue
Positive IF/ICC detected inHeLa cells, U-251 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66614-1-Ig targets PPP3CB in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PPP3CB fusion protein Ag26987 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 3 (formerly 2B), catalytic subunit, beta isoform
Calculated molecular weight 525 aa, 59 kDa
Observed molecular weight 59 kDa
GenBank accession numberBC028049
Gene Symbol PPP3CB
Gene ID (NCBI) 5532
RRIDAB_2881974
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP16298
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PPP3CB is one of the three isoforms of the A subunit of calcineurin (CnA). Calcineurin is a calcium-dependent serine/threonine phosphatase. It consists of two subunits: subunit A (CnA) and subunit B (CnB). For CnA, three isoforms PPP3CA (CnAα), PPP3CB (CnAβ) and PPP3CC (CnAγ) have been identified. PPP3CB is a calcium-dependent, calmodulin-stimulated protein phosphatase. It may have a role in the calmodulin activation of calcineurin.

Protocols

Product Specific Protocols
WB protocol for PPP3CB antibody 66614-1-IgDownload protocol
IF protocol for PPP3CB antibody 66614-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Autophagy

Narirutin activates TFEB (transcription factor EB) to protect against acetaminophen-induced liver injury by targeting PPP3/calcineurin

Authors - Zhiyuan Fang
  • KD Validated
View Article