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  • Featured Product
  • KD/KO Validated

PPP3CB Monoclonal antibody, PBS Only

PPP3CB Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3C1A3

Cat no : 66614-1-PBS

Synonyms

CALNA2, CAM-PRP catalytic subunit, CAM PRP catalytic subunit, CALNB, Calmodulin-dependent calcineurin A subunit beta isoform

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPP3CB Monoclonal antibody (66614-1-Ig)

    WB analysis using 66614-1-Ig (same clone as 66614-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66614-1-Ig (PPP3CB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66614-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using PPP3CB Monoclonal antibody (66614-1-Ig)

    WB analysis of rat brain using 66614-1-Ig (same clone as 66614-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66614-1-Ig (PPP3CB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66614-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using PPP3CB Monoclonal antibody (66614-1-Ig)

    WB analysis of mouse brain using 66614-1-Ig (same clone as 66614-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66614-1-Ig (PPP3CB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66614-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using PPP3CB Monoclonal antibody (66614-1-Ig)

    WB analysis of NIH/3T3 using 66614-1-Ig (same clone as 66614-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66614-1-Ig (PPP3CB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66614-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of fetal human brain tissue using PPP3CB Monoclonal antibody (66614-1-Ig)

    WB analysis of fetal human brain using 66614-1-Ig (same clone as 66614-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66614-1-Ig (PPP3CB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66614-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PPP3CB Monoclonal antibody (66614-1-Ig)

    IF Staining of HeLa using 66614-1-Ig (same clone as 66614-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PPP3CB antibody (66614-1-Ig, Clone: 3C1A3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66614-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using PPP3CB Monoclonal antibody (66614-1-Ig)

    IF Staining of U-251 using 66614-1-Ig (same clone as 66614-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using PPP3CB antibody (66614-1-Ig, Clone: 3C1A3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66614-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66614-1-PBS targets PPP3CB in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PPP3CB fusion protein Ag26987 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 3 (formerly 2B), catalytic subunit, beta isoform
Calculated molecular weight 525 aa, 59 kDa
Observed molecular weight 59 kDa
GenBank accession numberBC028049
Gene symbol PPP3CB
Gene ID (NCBI) 5532
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PPP3CB is one of the three isoforms of the A subunit of calcineurin (CnA). Calcineurin is a calcium-dependent serine/threonine phosphatase. It consists of two subunits: subunit A (CnA) and subunit B (CnB). For CnA, three isoforms PPP3CA (CnAα), PPP3CB (CnAβ) and PPP3CC (CnAγ) have been identified. PPP3CB is a calcium-dependent, calmodulin-stimulated protein phosphatase. It may have a role in the calmodulin activation of calcineurin.