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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPP3CB Monoclonal antibody (66614-1-Ig)

    WB analysis using 66614-1-Ig (same clone as 66614-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66614-1-Ig (PPP3CB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66614-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using PPP3CB Monoclonal antibody (66614-1-Ig)

    WB analysis of rat brain using 66614-1-Ig (same clone as 66614-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66614-1-Ig (PPP3CB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66614-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using PPP3CB Monoclonal antibody (66614-1-Ig)

    WB analysis of mouse brain using 66614-1-Ig (same clone as 66614-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66614-1-Ig (PPP3CB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66614-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using PPP3CB Monoclonal antibody (66614-1-Ig)

    WB analysis of NIH/3T3 using 66614-1-Ig (same clone as 66614-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66614-1-Ig (PPP3CB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66614-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of fetal human brain tissue using PPP3CB Monoclonal antibody (66614-1-Ig)

    WB analysis of fetal human brain using 66614-1-Ig (same clone as 66614-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66614-1-Ig (PPP3CB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66614-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PPP3CB Monoclonal antibody (66614-1-Ig)

    IF Staining of HeLa using 66614-1-Ig (same clone as 66614-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PPP3CB antibody (66614-1-Ig, Clone: 3C1A3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66614-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using PPP3CB Monoclonal antibody (66614-1-Ig)

    IF Staining of U-251 using 66614-1-Ig (same clone as 66614-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using PPP3CB antibody (66614-1-Ig, Clone: 3C1A3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66614-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

PPP3CB Monoclonal antibody, PBS Only

PPP3CB Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 66614-1-PBS
Clone No.3C1A3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

CALNA2, CAM-PRP catalytic subunit, CAM PRP catalytic subunit, CALNB, Calmodulin-dependent calcineurin A subunit beta isoform

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66614-1-PBS targets PPP3CB in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PPP3CB fusion protein Ag26987 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 3 (formerly 2B), catalytic subunit, beta isoform
Calculated molecular weight 525 aa, 59 kDa
Observed molecular weight 59 kDa
GenBank accession numberBC028049
Gene Symbol PPP3CB
Gene ID (NCBI) 5532
RRIDAB_2881974
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP16298
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PPP3CB is one of the three isoforms of the A subunit of calcineurin (CnA). Calcineurin is a calcium-dependent serine/threonine phosphatase. It consists of two subunits: subunit A (CnA) and subunit B (CnB). For CnA, three isoforms PPP3CA (CnAα), PPP3CB (CnAβ) and PPP3CC (CnAγ) have been identified. PPP3CB is a calcium-dependent, calmodulin-stimulated protein phosphatase. It may have a role in the calmodulin activation of calcineurin.