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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated PPP4R1 Monoclonal antibody

PPP4R1 Monoclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF-P, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3D12G12

Cat no : CL594-67392

Synonyms

Serine/threonine-protein phosphatase 4 regulatory subunit 1, PP4R1, PP4(Rmeg), MEG1, 3D12G12

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using CoraLite®594-conjugated PPP4R1 Monoclonal antibody (CL594-67392)

    IF Staining of mouse liver using CL594-67392

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CoraLite®594 PPP4R1 antibody (CL594-67392, Clone: 3D12G12 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated PPP4R1 Monoclonal antibody (CL594-67392)

    FC experiment of HeLa using CL594-67392

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human PPP4R1 (CL594-67392, Clone:3D12G12) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF-P detected inmouse liver tissue
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67392 targets PPP4R1 in IF-P, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PPP4R1 fusion protein Ag26750 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 4, regulatory subunit 1
Calculated molecular weight 107 kDa
Observed molecular weight 120-130 kDa
GenBank accession numberBC060829
Gene symbol PPP4R1
Gene ID (NCBI) 9989
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL594 PPP4R1 antibody CL594-67392Download protocol
FC protocol for CL594 PPP4R1 antibody CL594-67392Download protocol
Standard Protocols
Click here to view our Standard Protocols