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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PQBP1 Recombinant antibody (84491-6-RR)

    WB analysis using 84491-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84491-6-RR (PQBP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PQBP1 Recombinant antibody (84491-6-RR)

    IF Staining of HeLa using 84491-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PQBP1 antibody (84491-6-RR, Clone: 241901B11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84491-6-RR

    Affinity and Kinetic Characterization of 84491-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84491-6-RR against Human PQBP1 were performed. The affinity constant is 0.529 nM.

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PQBP1 Recombinant antibody

PQBP1 Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84491-6-RR
Clone No.241901B11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

PQBP-1, Polyglutamine-binding protein 1, Polyglutamine tract-binding protein 1, Npw38, 241901B11

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293T cells, mouse pancreas tissue, rat brain tissue, rat thymus tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84491-6-RR targets PQBP1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PQBP1 fusion protein Ag9329 相同性解析による交差性が予測される生物種
Full Name polyglutamine binding protein 1
Calculated molecular weight 265 aa, 30 kDa
Observed molecular weight 30-32 kDa
GenBank accession numberBC012358
Gene Symbol PQBP1
Gene ID (NCBI) 10084
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO60828
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PQBP1 (Polyglutamine-binding protein 1) is also named as NPW38 and JM26. PQBP1 senses extrinsic tau 3R/4R proteins by direct interaction and triggers an innate immune response by activating a cyclic GMP-AMP synthase (cGAS)-Stimulator of interferon genes (STING) pathway (PMID: 34782623).  PQBP1 is abundantly expressed in the central nervous system during development. PQBP1 is a component of the precatalytic spliceosome (B complex) that is involved in splicing. PQBP1 was predominantly localized in the cell nucleus. PQBP1 is overexpressed in various cancer types including breast cancer, colon cancer, and glioblastoma (PMID: 38342602).

Protocols

Product Specific Protocols
WB protocol for PQBP1 antibody 84491-6-RRDownload protocol
IF protocol for PQBP1 antibody 84491-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols