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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Biotin-conjugated PR Monoclonal antibody

PR Monoclonal Antibody for IHC

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC

Conjugate

Biotin

CloneNo.

3D1D12

Cat no : Biotin-66300

Synonyms

NR3C3, PGR, PR, progesterone receptor

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Biotin-conjugated PR Monoclonal antibody (Biotin-66300)

    IHC staining of human breast cancer using Biotin-66300

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using Biotin-66300 (PR antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Biotin-conjugated PR Monoclonal antibody (Biotin-66300)

    IHC staining of human ovary tumor using Biotin-66300

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using Biotin-66300 (PR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Biotin-conjugated PR Monoclonal antibody (Biotin-66300)

    IHC staining of human breast cancer using Biotin-66300

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using Biotin-66300 (PR antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive IHC detected inhuman ovary tumor tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-66300 targets PR in IHC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PR fusion protein Ag22992 相同性解析による交差性が予測される生物種
Full Name progesterone receptor
Calculated molecular weight 933 aa, 99 kDa
GenBank accession numberNM_000926
Gene symbol Progesterone Receptor
Gene ID (NCBI) 5241
RRIDAB_2883069
Conjugate Biotin
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PGR, also named as NR3C3 and PR, belongs to the nuclear hormone receptor family and NR3 subfamily. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation. The ER and PR status has been used as a predictor of breast carcinoma responsiveness to endocrine therapy and as a prognostic indicator for early recurrence.

Protocols

Product Specific Protocols
IHC protocol for Biotin PR antibody Biotin-66300Download protocol
Standard Protocols
Click here to view our Standard Protocols