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  • Featured Product
  • KD/KO Validated

PRAME Monoclonal antibody

PRAME Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1E9G9

Cat no : 68097-1-Ig

Synonyms

MAPE, OIP 4, OIP4, Opa interacting protein 4, PRAME

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PRAME Monoclonal antibody (68097-1-Ig)

    WB analysis using 68097-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68097-1-Ig (PRAME antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PRAME Monoclonal antibody (68097-1-Ig)

    WB analysis of HeLa using 68097-1-Ig

    WB result of PRAME antibody (68097-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRAME transfected HeLa cells.

  • Western Blot (WB) analysis of rat testis tissue using PRAME Monoclonal antibody (68097-1-Ig)

    WB analysis of rat testis using 68097-1-Ig

    rat testis tissue were subjected to SDS PAGE followed by western blot with 68097-1-Ig (PRAME antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using PRAME Monoclonal antibody (68097-1-Ig)

    WB analysis of mouse testis using 68097-1-Ig

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 68097-1-Ig (PRAME antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PRAME Monoclonal antibody (68097-1-Ig)

    WB analysis of HeLa using 68097-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 68097-1-Ig (PRAME antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using PRAME Monoclonal antibody (68097-1-Ig)

    WB analysis of A375 using 68097-1-Ig

    A375 cells were subjected to SDS PAGE followed by western blot with 68097-1-Ig (PRAME antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HaCaT cells using PRAME Monoclonal antibody (68097-1-Ig)

    IF Staining of HaCaT using 68097-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HaCaT cells using PRAME antibody (68097-1-Ig, Clone: 1E9G9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inA549 cells, HeLa cells, A375 cells, mouse testis tissue, K-562 cells, U2OS cells
Positive IF/ICC detected inHaCaT cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68097-1-Ig targets PRAME in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen PRAME fusion protein Ag1906 相同性解析による交差性が予測される生物種
Full Name preferentially expressed antigen in melanoma
Calculated molecular weight 509 aa, 58 kDa
Observed molecular weight50 kDa
GenBank accession numberBC014074
Gene symbol PRAME
Gene ID (NCBI) 23532
RRIDAB_2918834
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The PRAME (preferentially expressed antigen of melanoma) gene was previously shown to be overexpressed in ovarian/primary peritoneal serous carcinoma compared with malignant mesothelioma using gene expression arrays. It is considered a melanocyte differentiation antigen which is overexpressed in both solid and hematologic tumors. In normal tissue, a very low level of PRAME expression is found in normal testis, adrenals, ovary and endometrium. A high level of PRAME expression has been reported for several solid tumors, including ovarian cancer, breast cancer, lung cancer and melanomas, medulloblastoma, sarcomas, head and neck cancers, neuroblastoma, renal cancer, and Wilms'tumor. As a nuclear transcriptional repressor protein, PRAME binds to retinoic acid receptor a, thereby inhibiting retinoic acid induced differentiation, growth arrest, and apoptosis.

Protocols

Product Specific Protocols
WB protocol for PRAME antibody 68097-1-IgDownload protocol
IF protocol for PRAME antibody 68097-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols