CoraLite® Plus 488-conjugated PRDX3 Recombinant antibody
PRDX3 Recombinant Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1L11
Cat no : CL488-81833
Synonyms
Validation Data Gallery
Tested Applications
Positive FC (Intra) detected in | MCF-7 cells |
Recommended dilution
Application | Dilution |
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Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL488-81833 targets PRDX3 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | PRDX3 fusion protein Ag1062 相同性解析による交差性が予測される生物種 |
Full Name | peroxiredoxin 3 |
Calculated molecular weight | 27 kDa |
Observed molecular weight | 28 kDa |
GenBank accession number | BC007062 |
Gene symbol | PRDX3 |
Gene ID (NCBI) | 10935 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PRDX3(Peroxiredoxin 3), also named as AOP1, HBC189 and MER5, belongs to the ahpC/TSA family. It is involved in redox regulation of the cell and protects radical-sensitive enzymes from oxidative damage by a radical-generating system. PRDX3 is required for MYC-mediated proliferation, transformation, and apoptosis after glucose withdrawal and essential for maintaining mitochondrial mass and membrane potential in transformed rat and human cells (PMID:12011429). PRDX3 acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol.
Protocols
Product Specific Protocols | |
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FC protocol for CL Plus 488 PRDX3 antibody CL488-81833 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |