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PRDX4 Polyclonal antibody

Name: PRDX4 Polyclonal antibody
Brand: Proteintech
SKU: 10703-1-AP

PRDX4 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10703-1-AP

Synonyms

Peroxiredoxin IV, peroxiredoxin 4, EC:1.11.1.24, AOE37-2, AOE37 2

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 10703-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10703-1-AP (PRDX4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 10703-1-AP
WB result of PRDX4 antibody (10703-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRDX4 transfected HepG2 cells.
IP experiment of HepG2 using 10703-1-AP
IP result of anti-PRDX4 (IP:10703-1-AP, 4ug; Detection:10703-1-AP 1:4000) with HepG2 cells lysate 2040 ug.
IHC staining of human colon cancer using 10703-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10703-1-AP (PRDX4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 10703-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10703-1-AP (PRDX4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 10703-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10703-1-AP (PRDX4 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human colon cancer using 10703-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10703-1-AP (PRDX4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 10703-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PRDX4 antibody (10703-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HepG2 using 10703-1-AP
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human PRDX4 (10703-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 10703-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A375 cells, HepG2 cells, HEK-293 cells
Positive IP detected in	HepG2 cells
Positive IHC detected in	human colon cancer tissue, human pancreas cancer tissue, human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 21 publications below
IHC	See 5 publications below
IF	See 7 publications below
IP	See 2 publications below

Product Information

10703-1-AP targets PRDX4 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse, rat, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PRDX4 fusion protein Ag1177 相同性解析による交差性が予測される生物種
Full Name	peroxiredoxin 4
Calculated molecular weight	31 kDa
Observed molecular weight	30 kDa
GenBank accession number	BC007107
Gene symbol	PRDX4
Gene ID (NCBI)	10549
RRID	AB_2168493
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PRDX4 (Peroxiredoxin-4) is also named as AOE37-2, Prx-IV and belongs to the AhpC/TSA family. PRDX4 is associated with acrosome formation during rat spermatogenesis and has a protective role in the male reproductive tract, because the phenotype of mice lacking this isoform includes testicular atrophy and increased sperm DNA damage (PMID:20864641). It is initially synthesized as a membrane-binding 31-kDa protein and processed into a 27-kDa secretory form and is discarded with the residual bodies (PMID:19208552). PRDX4 is a pentamer of dimers (PMID:23025503). This antibody is specific to PRDX4.

Protocols

Product Specific Protocols
WB protocol for PRDX4 antibody 10703-1-AP	Download protocol
IHC protocol for PRDX4 antibody 10703-1-AP	Download protocol
IF protocol for PRDX4 antibody 10703-1-AP	Download protocol
IP protocol for PRDX4 antibody 10703-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IP	Nat Commun The deubiquitinase OTUD1 regulates immunoglobulin production and proteasome inhibitor sensitivity in multiple myeloma Authors - Alexander Vdovin View Article
rat	WB	Br J Pharmacol Luteolin ameliorates rat myocardial ischemia-reperfusion injury through peroxiredoxin II activation. Authors - Bo Wei View Article
human	WB	Antioxid Redox Signal Pro-Apoptotic Effects of JDA-202, a Novel Natural Diterpenoid, on Esophageal Cancer Through Targeting Peroxiredoxin I. Authors - Xiao-Jing Shi View Article
human	WB	Antioxid Redox Signal Direct Proteomic Mapping of Cysteine Persulfidation. Authors - Ling Fu View Article
mouse	WB	Bioorg Chem Novel [1,2,3]triazolo[4,5-d]pyrimidine derivatives containing hydrazone fragment as potent and selective anticancer agents. Authors - Chenhao Xu View Article
	WB,IHC,IF,IP	FEBS J A guide to assessing endoplasmic reticulum homeostasis and stress in mammalian systems. Authors - Daria Sicari View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PRDX4 Polyclonal antibody

PRDX4 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Validation Data Gallery

WB analysis using 10703-1-AP

WB analysis of HepG2 using 10703-1-AP

IP experiment of HepG2 using 10703-1-AP

IHC staining of human colon cancer using 10703-1-AP

IHC staining of human liver cancer using 10703-1-AP

IHC staining of human pancreas cancer using 10703-1-AP

IHC staining of human colon cancer using 10703-1-AP

IF Staining of HepG2 using 10703-1-AP

FC experiment of HepG2 using 10703-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

The deubiquitinase OTUD1 regulates immunoglobulin production and proteasome inhibitor sensitivity in multiple myeloma

Luteolin ameliorates rat myocardial ischemia-reperfusion injury through peroxiredoxin II activation.

Pro-Apoptotic Effects of JDA-202, a Novel Natural Diterpenoid, on Esophageal Cancer Through Targeting Peroxiredoxin I.

Direct Proteomic Mapping of Cysteine Persulfidation.

Novel [1,2,3]triazolo[4,5-d]pyrimidine derivatives containing hydrazone fragment as potent and selective anticancer agents.

A guide to assessing endoplasmic reticulum homeostasis and stress in mammalian systems.